Fluorolog-3 v. 2.2 (10 Sep 2002)
Components & Accessories
6-5
Note:
If the detectors are
saturated, close the slits a
little or install a neutral-
density filter.
10
Adjust the high voltage HV2, as needed, so that
the reference signal is ~ 1 µA (saturation of the
reference detector is 10 µA).
11
Acquire transmission spectra.
Either
a
Position the emission spectrometer at 0 nm, and use the
S/R
acquisition
mode.
b
Acquire an excitation scan of the blank.
c
Acquire and excitation scan of the sample.
Or
When the spectrometers are synchronously scanned using an offset of 0 nm, the
emission slits should be opened five times as wide as the slits of the excitation
spectrometer.
d
Acquire a synchronous scan
with an offset of 0 nm over the
absorption range of the blank.
e
Acquire a synchronous scan
with an offset of 0 nm over the
absorption range of the
sample.
12
Calculate the absorption.
The absorption spectrum can be determined from the following equation:
A
= log(
S
/
R
)
blank
– log(
S
/
R
)
sample
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