Fluorolog-3 v. 2.2 (11 Jul 2002)
iii
Table of Contents
0: Introduction ................................................................................................0-1
About the Fluorolog
®
-3............................................................................................................................ 0-1
Chapter overview .................................................................................................................................... 0-2
Symbols used in this manual .................................................................................................................. 0-4
1: Requirements & Installation ............................................................................1-1
Surface requirements ............................................................................................................................. 1-1
Environmental requirements................................................................................................................... 1-2
Electrical requirements ........................................................................................................................... 1-3
Installation............................................................................................................................................... 1-4
2: System Description .......................................................................................2-1
Overview ................................................................................................................................................. 2-1
Configurations......................................................................................................................................... 2-3
3: System Operation .........................................................................................3-1
Turning on the system ............................................................................................................................ 3-1
Checking system performance ............................................................................................................... 3-3
Notes on excitation and emission recalibration ....................................................................................3-19
Useful materials for characterizing system and samples .....................................................................3-20
4: Optimizing Data Acquisition.............................................................................4-1
Cuvette preparation ................................................................................................................................ 4-1
Sample preparation ................................................................................................................................ 4-2
Data collection techniques...................................................................................................................... 4-3
Correcting data .....................................................................................................................................4-18
5: System Maintenance .....................................................................................5-1
Lamp ....................................................................................................................................................... 5-1
Emission signal detector.......................................................................................................................5-14
Reference signal detector.....................................................................................................................5-15
Gratings ................................................................................................................................................5-16
Mirrors...................................................................................................................................................5-20
Automated 4-position turret ..................................................................................................................5-21
6: Components & Accessories .............................................................................6-1
Itemized list ............................................................................................................................................. 6-2
Model 1940 absorption/transmission accessory..................................................................................... 6-3
FL-1013 Liquid nitrogen Dewar assembly .............................................................................................. 6-6
Model 1908MOD scatter block assembly ............................................................................................... 6-7
Model 1908 standard lamp assembly..................................................................................................... 6-7
Sample cells............................................................................................................................................ 6-8
Model 1967 Photodiode reference detector ........................................................................................... 6-9
CCD detectors ......................................................................................................................................6-10
Model 1911F room temperature signal detector...................................................................................6-11
Model 1914F thermoelectrically cooled signal detector........................................................................6-12
FL-1030 thermoelectrically cooled near-IR photomultiplier tube ..........................................................6-13
F-3000 fiber optic mount.......................................................................................................................6-14
Model 1938 cut-on filter ........................................................................................................................6-15
Model 1939 cut-on filter ........................................................................................................................6-15
Fl-1001 Front-face viewing option ........................................................................................................6-16
Gratings ................................................................................................................................................6-17
FL-1010 cut-on filter holder...................................................................................................................6-18
FL-1011 four-position thermostatted cell holder ...................................................................................6-19
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