Fluorolog-3 v. 2.2 (10 Sep 2002)
Optimizing Data Acquisition
4-4
Note:
If you have not checked instrument performance, Jobin
Yvon Inc. recommends acquiring a lamp spectrum and a wa-
ter Raman spectrum, as outlined in the previous chapter, be-
fore proceeding.
Note:
Make sure the lid
is completely closed.
Note:
The default volt-
ages will be used if the
value is not changed.
Preliminary emission maximum
1
Verify that all system components are on.
2
Place the sample in
the sample
compartment.
3
Enter the
Real Time
Display
:
4
Turn on the high
voltage to the signal
detector.
5
Set the excitation
and emission slits to
a bandpass of 5.0
nm.
After the value is entered, hit the
Tab
key on the keyboard to set the slits.
6
Close the
Real Time Display
.
The objective is to acquire a preliminary emission scan using an
approximate excitation wavelength. Once the emission peak is determined,
this value will be used to obtain the optimal excitation spectrum.
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