Fluorolog-3 v. 2.2 (10 Sep 2002)
Components & Accessories
6-4
Warning:
Only when both emission and reference signals are within
prescribed limits should you proceed with the measurements.
Note:
If uncertain of the absorption range, enter a range of 250 nm to
400 nm, and scan the excitation spectrometer. Detailed instructions
for setting parameters are contained in the software manual. The
S/R
acquisition mode compensates for fluctuations in lamp intensity,
and wavelength dependency of the lamp and excitation spectrome-
ter.
Note:
The linear range of the
R928P detector operated in
the photon-counting mode is
2 million cps.
2
Place the cuvette containing the blank in the
sample holder.
3
Set the emission spectrometer position to 0 nm.
4
Select acquisition mode
S/R
.
5
Run an excitation scan over the absorption
range of the sample.
6
Note the emission signal at maximum
transmission of the blank.
7
Adjust the slits to
maximize the
signal without
saturating the
detector.
8
Similarly, locate the maximum reference signal
of the lamp spectrum.
9
Set the excitation spectrometer to that position.
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
