Fluorolog-3 v. 2.2 (11 Jul 2002)
iv
FL-1012 dual-position thermostatted cell holder .................................................................................. 6-21
Model 1933 solid sample holder........................................................................................................... 6-23
FL-1039 xenon lamp housing ............................................................................................................... 6-25
FL-1040 dual lamp housing .................................................................................................................. 6-25
F-3005/6 autotitration injector............................................................................................................... 6-26
Models F-3001, F-3002, and F-3003 microscope interfaces ............................................................... 6-27
Model 1907 450-W xenon lamp............................................................................................................ 6-28
F-3004 sample heater/cooler Peltier thermocouple drive..................................................................... 6-29
Phosphorimeter accessory ................................................................................................................... 6-30
MicroMax microwell plate reader.......................................................................................................... 6-31
FL-1044 L-format polarizer & FL-1045 T-format polarizer.................................................................... 6-32
FL-1015 injector port............................................................................................................................. 6-33
F-1000/1 temperature bath................................................................................................................... 6-34
Model TRIG-15/25 external trigger accessory...................................................................................... 6-35
7: Troubleshooting........................................................................................... 7-1
Using diagnostic spectra ........................................................................................................................ 7-3
Further assistance…............................................................................................................................... 7-8
8: Introduction to lifetime measurements ............................................................... 8-1
Introduction ............................................................................................................................................. 8-1
Lifetime measurements .......................................................................................................................... 8-2
Types of lifetime scans ........................................................................................................................... 8-4
9: Xenon Lamp Information & Record of Use Form..................................................... 9-1
Xenon lamp record of use ...................................................................................................................... 9-3
10: Applications.............................................................................................10-1
Introduction ........................................................................................................................................... 10-1
Detecting sub-picomolar concentrations of fluorescein........................................................................ 10-3
Reduced-volume samples .................................................................................................................... 10-3
Fluorescence detection of highly scattering samples........................................................................... 10-4
Quantum-yield calculations .................................................................................................................. 10-4
Characterizing complex mixtures via synchronous scanning............................................................... 10-4
Operating in the IR region .................................................................................................................... 10-5
Phosphorescence for time-resolved data............................................................................................. 10-5
Low-temperature scans ........................................................................................................................ 10-6
Monitoring kinetic reactions using time-based fluorescence................................................................ 10-6
Front-face detection to enhance data collection for absorbent or solid samples ................................. 10-6
Polarization to detect trace quantities of biological probes .................................................................. 10-7
11: Producing Correction Factors ........................................................................11-1
Introduction ........................................................................................................................................... 11-1
Generating emission correction factors................................................................................................ 11-2
Calculating emission correction factors................................................................................................ 11-8
Calculating excitation correction factors............................................................................................. 11-15
12: Determining the Plateau Voltage ....................................................................12-1
Introduction ........................................................................................................................................... 12-1
Overview of the procedure ................................................................................................................... 12-1
Procedure ............................................................................................................................................. 12-3
13: Reassemby Instructions...............................................................................13-1
Computer .............................................................................................................................................. 13-1
Spectrofluorometer assembly............................................................................................................... 13-2
Cable connections ................................................................................................................................ 13-3
Connecting power cables ..................................................................................................................... 13-7
14: Using TRIAX with the Fluorolog
®
-3..................................................................14-1
Introduction ........................................................................................................................................... 14-1
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