Fluorolog-3 v. 2.2 (9 Aug 2002)
TRIAX operation with the Fluorolog
®
-3
14-1
14: TRIAX operation with the
Fluorolog
®
-3
Introduction
Some users choose the TRIAX series of imaging spectrometers as a building block in a
Fluorolog
®
-3 spectrofluorometer. Using a TRIAX imaging spectrometer on the emis-
sion side of the sample mount offers the option of detection with a CCD, to create an
image of the dispersed fluorescence for subsequent analysis. This chapter discusses
special hardware connections and software operation specific to TRIAX users, espe-
cially with regard to CCD detectors.
Fluorolog
®
-3 TRIAX users should note these major differences between the TRIAX
and standard monochromators:
•
Four ports generally are available, rather than two on a standard monochromator.
Most TRIAX spectrometers used with the Fluorolog
®
-3 have two entrance ports
(“lateral”, i.e., on the side, and “axial”, i.e., on the back) and two exit ports (lateral
and axial). Usually the lateral entrance port is attached to the sample compartment.
•
A DataMax-controlled flip mirror is available to
choose between entrance ports and exit ports. Be sure
the flip mirror is set to the proper position before
running an experiment.
•
Three gratings can be mounted on the rotatable grating
turret. Choosing which grating to use is under
DataMax’s control.
Lateral entrance port (with
baseplate)
Axial entrance
Lateral exit port with PMT
Axial exit port
with CCD
Flip mirror in
axial position
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