Fluorolog-3 v. 2.2 (31 Jul 2002)
Producing Correction Factors
11-6
Determine the dark counts
1
Place the sample lid over the mask to block light
to the detector.
2
In
Run Experiment
, select the Experiment button.
This opens the
Emission Acquisition
dialog box.
3
Type in the following parameters in the
Emission
Acquisition
dialog box:
Number of Scans
1
Start 290
nm
End 850
nm
Increment
5
nm
Integration Time
1 s
Excitation Position
350 nm
4
Run the standard lamp spectrum.
5
Name this file
stlamp
.
6
Place the lid over the mask to block the light
between the standard lamp and the scatter
fixture.
7
Run another scan using the same parameters,
and name this file
blank
.
This data file is a straight line with low intensity.
8
Choose Arithmetic from the toolbar.
9
Using the Arithmetic menu, subtract
blank
from
stdlamp
.
10
Name this file
stdlamp2
.
Below are the blank and lamp spectra. Notice that the blank is almost non-
existent. Because of the low intensity of the blank file, the blank-subtracted file,
stdlamp2
, will resemble the
stdlamp
file.
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
