Fluorolog-3 v. 2.2 (11 Jul 2002)
Applications
10-1
10: Applications
Introduction
The Fluorolog
®
series of spectrofluorometers have earned the reputation for being the
most sensitive in the world. Because the Fluorolog
®
-3 provides superior optical and
electronic components combined with a comprehensive selection of accessories, it pro-
vides the highest sensitivity and selectivity for all types of samples.
Jobin Yvon Inc. realizes the importance of accurate and reproducible data. Therefore,
the systems are calibrated and tested prior to shipment to a customer site and then again
after installation. Performance spectra indicate the operational parameters and ensure
that the system is operating within specifications. Fluorolog
®
-3 systems employ the
most sophisticated data-correction techniques to yield accurate and reproducible fluo-
rescence spectra.
For example, fluorescence emission spectra are affected by the response characteristics
of the spectrometer and optical components such as gratings and detectors. To compen-
sate for these responses, radiometric correction factors are individually determined for
each Fluorolog
®
-3 system and are supplied with the DataMax software. The user can
update these correction factors periodically if necessary. Simple point-and-click opera-
tions or user-controllable default system parameters allow real-time or post-processing
correction of spectral data.
To correct for variations in the intensity of the excitation source, Fluorolog
®
-3 spectro-
fluorometers also monitor the excitation beam with a wavelength-independent refer-
ence detector. The raw emission data can then be automatically ratioed to intensity in-
formation generated by the reference detector. The effect of this correction can be seen
in the upper plot on the next page.
Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com
