Fluorolog-3 v. 2.2 (31 Jul 2002)
Troubleshooting
7-5
Water Raman spectra
Contaminated water
Running a water Raman scan helps identify abnormalities as a result of accessory prob-
lems or miscalibration. The following spectrum is normal:
Below is a normal water Raman spectrum superimposed on one that exhibits a problem.
In this instance, the water was contaminated, resulting in a high background.
If a spectrum similar to this is obtained after running a water Raman scan,
1
Rotate the cuvette 90° and rerun the scan.
Clean water Raman scan.
0
1
2
3
4
5
365
380
395
410
425
440
455
Wavelength (nm)
Intensity (10
5
counts/s)
397 nm
C o n tam in ated w ater in a w ater R am an scan .
0
1
2
3
4
5
3 6 5
3 8 0
3 9 5
4 1 0
4 2 5
4 4 0
4 5 5
W a v e le ngth (nm)
Intensity (10
5
counts/s)
Contaminated water
(note high background
)
Clean water
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