Fluorolog-3 v. 2.2 (11 Jul 2002)
System Operation
3-15
Note:
Observed throughput (and hence peak
intensity) is affected by lamp age and align-
ment, slit settings, and sample purity. As the
xenon lamp ages, the throughput of the sys-
tem will decline slowly. Therefore, low water
Raman peak intensity may indicate a need
to replace the xenon lamp.
The Rayleigh scatter band
or excitation band, which is
about 10 times the intensity
of the Raman band, occurs
at 350 nm (the excitation
wavelength). The scan
begins at 365 in order to
avoid detecting the Rayleigh
band.
4
Find the
peak of the water Raman band.
Is the peak at
397 ± 0.5 nm?
Instrument is within
specification.
Calibration is com-
plete.
Instrument is
not
within
specification.
No
Yes
Has the excitation
monochromator
been calibrated?
Continue on to
Emission recali-
bration.
Yes
Go back to Excitation
calibration.
No
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