Fluorolog-3 v. 2.2 (10 Sep 2002)
Components & Accessories
6-15
Model 1938 Cut-On Filter
The Model 1938 Cut-On Filter Set consists of 5 filters with dimensions of 1" × 2". To
properly position
the filter, the
Model FL-1010
Filter Holder is
required.
Cut-on filters are
used to eliminate
second-order effects of the gratings. For example, if sample excitation is at 300 nm, a
second-order peak occurs at 600 nm. If the emission spectrum extends from 400 nm to
650 nm, a sharp spike occurs at 600 nm. This peak is the second-order peak of the exci-
tation spectrometer. To remove this unwanted peak in the emission spectrum, place a
350-nm filter in the emission slot. Cut-on filters typically are used for phosphorescence
measurements, where second-order effects are likely to be found.
The single-beam and T-box sampling compartments have three slots that can hold the
Model FL-1010 filter holder. One slot is in the excitation light path and the other two
are the emission light path positions. To eliminate second-order effects from an excita-
tion spectrum, install the filter holder and the appropriate cut-on filter in the excitation
light path.
Model 1939 Cut-On Filter
The Model 1939 Cut-On Filter set consists of five 2" × 2" filters with cut-on wave-
lengths of 350 nm, 399 nm, 450 nm, 500 nm, and 550 nm. To properly position the fil-
ter, the FL-1010 Filter Holder is required.
Cut-on filters are used to eliminate second-order effects of the gratings. For example, if
sample excitation is at 300 nm, a second-order peak occurs at 600 nm. If the emission
spectrum extends from 400 nm to 650 nm, a sharp spike occurs at 600 nm. This peak is
the second-order peak of the excitation spectrometer. To remove this unwanted peak in
the emission spectrum, place a 350-nm filter in the emission slot. Cut-on filters are
typically used for phosphorescence measurements, where second-order effects are
likely to be found.
The single-beam and T-box sampling compartments have three slots that can hold the
FL-1010 filter holder. One slot is in the excitation light path and the other two are the
emission light path positions. To eliminate second-order effects from an excitation
spectrum, install the filter holder and the appropriate cut-on filter in the excitation light
path.
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