Fluorolog-3 v. 2.2 (11 Jul 2002)
System Description
2-1
Controller
2: System Description
Overview
General operation
All Fluorolog
®
-3 spectrofluorometers have common features:
a
A source of
radiation
produces
photons.
b
The beam of
light is
filtered by an
excitation
spectrometer
that allows a
single
wavelength
of light to
reach the
sample.
c
In the sample
compartment, the sample responds to the incoming radiation.
d
The resulting radiation is filtered by an emission spectrometer that feeds the
signal to a photomultiplier detector.
e
By stepping either or both spectrometers through a wavelength region, and
recording the variation in intensity as a function of wavelength, a spectrum
is produced.
f
The spectrofluorometer components (spectrometers, sample-compartment
module, accessories) are connected to a controller which, in turn, transfers
information to and from the computer. The computer may be attached to a
printer or plotter.
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