Fluorolog-3 v. 2.2 (31 Jul 2002)
Producing Correction Factors
11-2
Generating emission correction factors
Required kits
Emission correction factors should be updated periodically or whenever different grat-
ings or signal detectors are installed. The correction factors can be updated either at the
user’s location, or by a representative from the Spex
®
Fluorescence Service Depart-
ment. To arrange for a visit and a fee estimate, call our service department. To update
the correction factors without a service visit, follow the instructions below.
One way to generate correction factors for your instrument is to scan the spectrum of a
standard lamp. Because the actual irradiance values of the standard lamp as a function
of wavelength are known, dividing the irradiance values by the lamp spectrum results
in a set of relative correction values. These values can then be applied to the raw fluo-
rescence data. The emission correction factor file
mcorrect
was acquired in this
manner.
To generate emission correction factors, several items are needed: a standard lamp, ap-
propriate holders, and a scatter assembly. Jobin Yvon Inc. offers two kits: the
Model 1908 Standard Lamp Accessory, and the Model 1908MOD Scatter Assembly.
The Model 1908 is a complete correction factor kit, while the Model 1908MOD Scatter
Assembly is provided for users who already have a calibrated standard lamp and a con-
stant-current source.
The Model 1908 Standard Lamp Assembly is a complete correction factor kit, which
includes the following items:
•
200-watt quartz tungsten-halogen filament lamp with irradiance values
•
Constant Current Power Supply with lamp holder
•
1908MOD Scatter Assembly
•
The Model 1908MOD Scatter Assembly includes:
•
Lamp Mount Assembly and Mask with Square Center
•
Scatter Block with neutral-density filter and reflectance plate
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