Fluorolog-3 v. 2.2 (10 Sep 2002)
Optimizing Data Acquisition
4-17
Smoothing data
Smoothing the data improves the appearance of your spectrum. Smoothing, as are most
post-processing features, is handled by GRAMS/32
®
. By selecting
Arithmetic
from the
main menu of
Run Experiment
, you can choose
FFT
(fast-Fourier transform),
Binomial
, or
Savitsky-Golay
smoothing. Automatic smoothing and the degree to which smoothing oc-
curs can be achieved by entering the
Peaks/Settings
dialog box and typing the relevant in-
formation. Refer to the
GRAMS/32
®
User’s Guide
for additional information regarding
smoothing data.
An additional option,
Zap
, for removing outliers, also appears under the
Arithmetic
menu.
The
GRAMS/32
®
User’s Guide
covers this option thoroughly.
In general, start with a 9- or 11-point smooth for a time-base measurement. To select the
proper number of points for wavelength scan types, first locate the area that requires
smoothing—usually this is a peak. Determine the number of data points used to make up the
peak and then smooth the data using the number of points closest to this number. To avoid
artificially enhancing the data, use the appropriate number of points to smooth the data. For
example, selection of too large a number results in the background being smoothed into the
peak.
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