Fluorolog-3 v. 2.2 (11 Jul 2002)
System Operation
3-4
Excitation calibration check
This calibration check verifies the wavelength calibration of your excitation monochroma-
tor, using the reference photodiode located before the sample compartment. It is an excita-
tion scan of the xenon lamp’s output, and should be the first check performed.
1
Secure the lid of the sample chamber in place.
2
On the
Run Experiment
toolbar, select the
Experiment button.
This opens the
Emission Acquisition
dialog box:
3
Click on the Exp
Type… button.
This opens the
Select
Experiment Type
dialog
box:
4
Choose Excitation
Acquisition.
Click
OK
to close the
Select Experiment Type
dialog box. The
Emission Acquisition
window
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