Fluorolog-3 v. 2.2 (9 Aug 2002)
TRIAX operation with the Fluorolog
®
-3
14-36
Note:
Before applying correction factors, Jobin Yvon Inc. recom-
mends subtracting the dark counts, and the spectrum of the blank,
from the data. Refer to the software manual for details.
Warning:
DataMax only uses and displays the first 8 characters of a file
name. Therefore, the rest of a correction-factor file name may be trun-
cated, indicated by a tilde (~).
Warning:
For CCD arrays, these correction-factor files are only valid un-
der the same conditions, i.e., when used at the same central wave-
length as performed in the factory. The files may give invalid corrections
when used at other central wavelengths on the CCD array
.
Here are some examples of deciphering the emission correction-factor file:
Mcorrect.spc
The default emission correction-factor file for an instru-
ment, with no TRIAX, with only one emission grating and
detector.
MC9286x300.spc
An emission correction-factor file for an R928P PMT, with
a grating of 600 grooves/mm and a blaze at 300 mm.
MCCD12x500.spc
An emission correction-factor file for a CCD array, with a
grating of 1200 grooves/mm and a blaze at 500 nm.
MC26586x300.spc
An emission correction-factor file for an IR detector, with a
grating of 600 grooves/mm and a blaze at 300 nm.
Xcorrect.spc
The default excitation correction-factor file for an instru-
ment, with no TRIAX with only one excitation grating.
XC12x330.spc
An excitation correction-factor file for a grating with 1200
grooves/mm and a blaze at 330 nm.
To determine the center wavelength for a particular correction-factor file when used
with a CCD array, see the Performance Test Report that accompanies the instrument.
During acquisition
To acquire corrected emission data automatically, enter the appropriate emission cor-
rection-factor file,
mc…
, in the
Correction
factor file field in the
Run Experiment
dialog
box. To acquire corrected excitation data, enter the appropriate excitation correction-
factor file,
xc…
.
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