Fluorolog-3 v. 2.2 (31 Jul 2002)
Producing Correction Factors
11-8
Calculating emission correction factors
Introduction
Irradiance values for a standard lamp, packaged with the lamp, are usually expressed in
10
–6
W·cm
–2
·nm. With photon-counting systems like the Fluorolog
®
-3 spectrofluorome-
ters, however, data usually are collected in units of photons·s
–1
·cm
–2
·nm. To convert the
units, multiply each irradiance value by the wavelength at which it is valid. (The data
will still be off by a factor of
c
, but normalizing the correction factors compensates for
this.)
For more information about the theory and application of radiometric correction, con-
sult
Accuracy in Spectrophotometry and Luminescence Measurements
, Mavrodineau,
Schultz, and Menis, NBS Spec. Publ. 378 (1973), especially p. 137, “Absolute Spectro-
fluorometry,” by W.H. Melhuish.
Load the irradiance values
1
In
Run Experiment
, choose the
Experiment button
This opens the
Emission Acquisition
dialog box.
2
Click Exp Type.
This opens the
Select Experiment Type
dialog box:
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