Fluorolog-3 v. 2.2 (11 Jul 2002)
System Operation
3-12
Note:
Avoid glass or acrylic cuvettes: they
may exhibit UV fluorescence or filtering ef-
fects.
Note:
The emission calibration of the instrument is directly affected by the
calibration of the excitation monochromator.
Emission calibration check
This calibration check verifies the wavelength calibration of the emission monochromator
with the emission photomultiplier tube. It is an emission scan of the Raman-scatter band of
water performed in right-angle mode. This check should be performed after the xenon-lamp
scan. When completed, the performance of the system has been verified.
The water sample should be research-quality, triple-distilled or de-ionized water. HPLC-
grade (18-M
Ω
spec.) or equivalent water is suggested for the Raman scan. Impure samples
of water will cause elevated background levels as well as distorted spectra with (perhaps)
some unwelcome peaks.
Use a 4-mL quartz cuvette.
1
Insert the
water
sample into the sample compartment.
With an automated sample changer, note the position number in which the
sample cell is placed.
2
Make sure the lid of the sample chamber is
securely in place.
3
In the
Run Experiment
toolbar, choose the
Experiment button.
This opens the
Emission Acquisition
dialog box.
a
Click on
Exp Type…
to
open the
Select
Experiment Type
dialog
box:
Choose
Emission
Acquisition
. Click
OK
to
close the box.
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