Fluorolog-3 v. 2.2 (11 Jul 2002)
Applications
10-2
Spectra of azulene. Upper plot: corrected (solid line) and uncorrected (broken line)
excitation spectra. Lower plot: an absorption spectrum.
The broken line in the upper plot traces the uncorrected excitation spectrum of an az-
ulene sample acquired on a Fluorolog
®
system without reference-detector ratioing. The
solid line represents the same spectrum automatically corrected for the wavelength-
dependence of excitation-source intensity. The lower plot shows the absorption spec-
trum of azulene, acquired on the same spectrofluorometer system. The corrected excita-
tion spectrum shows strong similarity and fine structure compared with the correspond-
ing absorption spectrum.
The Fluorolog
®
series of spectrofluorometers have carved a niche in the scientific
community by consistently demonstrating the capability to perform extraordinary tasks.
Some of the remarkable features of the system are outlined below.
250 300 350 400
Wavelength (nm)
250 300 350 400
Wavelength (nm)
0.41
0.308
0.205
0.103
0
0.3
0.225
0.150
0.075
0
Intensity
Intensity
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