Fluorolog-3 v. 2.2 (31 Jul 2002)
Troubleshooting
7-1
7: Troubleshooting
The Fluorolog
®
-3 spectrofluorometer system has been designed to operate reliably and
predictably. Should a problem occur, examine the chart below, and try the steps listed
on the following pages.
Problem Possible
Cause
Remedy
Light is not reaching
the sample.
Excitation shutter closed.
Using the software, open the shutter.
Slits are not open to the
proper width.
Adjust the slits.
Lamp is not turned on
Turn on lamp by pressing lamp rocker switch,
and then the
Start
button on the xenon-lamp
power-supply front panel.
Monochromator is miscali-
brated.
Check and recalibrate monochromator.
Sample turret is not in cor-
rect position.
Using the software, set the position and open
the cover to verify the position.
Signal intensity is low.
Lamp is not aligned or
focused.
Align and focus the lamp.
Slits are not open to proper
width.
Adjust the slits.
Shutter(s) is(are) not com-
pletely open.
Open the shutter(s).
Lamp power supply is set
to the wrong current rating.
Call the Spex
®
Fluorescence Service Depart-
ment. (450-W Xe lamp current = 25 A.)
High voltage is improperly
set.
Enter proper voltage: Default HV1 = 950 V
Polarizer is in the light
path.
In
Visual Instrument Setup
, move the polar-
izer out of the light path.
Lamp is too old.
Replace lamp. (450-W lamp has lamp lifetime
1500–2000 h.)
No change in signal
intensity.
Shutter(s) closed.
Open all shutters.
Spectrometers are set to
wrong wavelength.
Select appropriate wavelength based on exci-
tation and emission of sample.
Detectors are saturated
Adjust slits and/or voltages. (Signal detector
has linearity to 1.5 × 10
6
cps when operated in
photon-counting mode.)
No signal.
Detectors are saturated.
Reduce slit settings.
High voltage is off.
Turn on high voltage through the software.
Erratic signal.
Lamp is not on.
Turn on lamp.
Light leaks.
Check dark value to determine.
[continued on next page]
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