Beckman Coulter Navios, Инструкция по эксплуатации

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Specifications
Performance Characteristics And Specifications
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Performance Characteristics And Specifications
NOTE
Unless stated otherwise, all performance characteristics and specifications were achieved at a
medium flow rate.
Carryover
Particle carryover is <0.1% from one specimen to another when the number of gated events is
10,000.
Fluorescence carryover on the Navios system was assessed by analyzing an unstained control
sample after the acquisition of a sample stained with a vital dye. Following the acquisition of three
tubes of Cyto-trol Control Cells stained with 20ul of Acridine Orange, the system was cleaned
according to the
Routine Cleaning Procedure . Subsequently, 3 tubes of unstained Cyto-trol Control
Cells were analyzed. The average mean channel fluorescence shift obtained in the unstained sample
acquisitions was less than 1%.
Acquisition Rate
The Navios was verified to analyze at least 200,000 events from FS, SS, FITC, PE, ECD, PC5.5, PC7, and
APC parameters with event rates from 1384 to 40822 events per second. Electronic pulses were
counted from the beads passing through the aperture as measured by discriminator events. The
count from the histogram display/printout was assessed against the number of pulses counted
(electronic count). Yield percentage was taken from the Stored Events counter. At 25,000 events per
second, the yield was measured to be 90%.
Data Acquisition Throughput
Throughput, including printing, of 80 tubes/hour of 10,000 normal leukocytes (~2,500
lymphocytes/uL) was obtained at a high flow rate. The throughput of a concentrated sample was 88
tubes/hour was obtained at 10,000 events per second when the stop count was set to 100,000 gated
events.
Acquisition throughput is dependant on many variables, including flow rate, data rate, number of
parameters collected, gated events, data plots, FlowPAGE reports and printouts generated. Changing
these variables, such as decreasing the number of events collected, increasing sample concentration
or increasing the flow rate can increase your acquisition throughput; while increasing the number
of events collected or running at a lower data rate can decrease your acquisition throughput.
Precision for Surface Markers
See reagent package insert for precision specifications of surface markers.