Olympus FLUOVIEW FV300, User Manual

Page: 58 / 564

Getting started FLUOVIEW/
Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV
. 1 - 2 0
Page
1-2 Outline of LSM Observation Procedures
x
Fluorescence observation procedure
Set the observation condition.
Start the system.
x
Turn the system power ON.
(Sec 1-2-1)
x
Start the FLUOVIEW software.
(Sec
1-2-2
)
x
Set the multiple sections to be
observed. (Sec 1-2-12-7)
x
Set the area to be observed.
(Sec 1-2-12-8)
x
Set a lower scan speed.
(Sec 1-2-12-9)
x
Stop repeated scanning.
(Sec 1-2-12-10)
If t
h
e ima
g
e
is
st
ill no
t d
ispla
ye
d
Select the LSM light path.
(Section 1-2-4)
Set the scan unit.
x
Set the DETECTION MODE
slider. (Sec 1-2-7)
x
Set the BARRIER FILTERS
slider. (Sec 1-2-8)
x
Set the Excitation DM. (Sec 1-2-9)
Select the light path for 100 binocular
tube and focus on the specimen.
(Section 1-2-3)
If no image is
displayed
If an image
is displayed
Adjust PMT Voltage.
(Sec 2-2-1-3-9)
Change the ND filter with a filter
with higher transmittance
(Sec 1-2-10)
When the combination using the laser
combiner is used, set the optimum ND
filters for the laser. (Sec 1-2-10)
x
Set the objective magnification.
(Sec 1-2-12-1)
x
Set the zoom ratio to 1X. (Sec 1-2-12-2)
x
Set the channels. (Sec 1-2-12-3)
x
Set the highest scan speed.
(Sec 1-2-12-4)
x
Select the XY observation mode.
(Sec 1-2-12-5)
x
Perform repeated scanning. (Sec 1-2-12-6)
x
Adjust the detection light axis. (Sec 1-2-13)
x
Set the CONFOCAL APERTURE turret.
(Sec1-2-6)
Image is
displayed in the
[Live] panel of the
software
Set the dyeing method.
(Sec 1-2-5)
Acquire an image.
(Sec 1-2-14)
Save the image.
(Sec 1-2-15)
Exit from the FLUOVIEW software.
(Sec 1-2-16)
Turn the system power OFF.
(Sec 1-2-17)