Olympus FLUOVIEW FV300, User Manual

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APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 9 8
Page
2-2-12-2 Example of FRAP exper iment
The example of procedure of FRAP experiment using AOTF is described in this section.
In order to perform photobleaching to a specimen, the value of laser intensity is raised to
100% to acquire an image since strong laser intensity is temporarily required. When
100% or more of laser intensity value is required, zoom magnification is gathered to
acquire an image. (It is because the square power of zoom magnification per time can
be secured.) Moreover, using the objective of larger NA concentrates the laser
irradiation spot in Z direction, and stronger laser can be irradiated.
Therefore, a preliminary experiment is required to set up the laser intensity, the objective,
the zoom ration of the REX mask file according to a specimen.
1 Preliminary Experiment
1. Set the laser intensity, the objective, and the zoom ration in the [Acquire] panel so
that they are suitable for a specimen.
2 Acquiring Image in XY m ode
1. Confirm that [XY-Norm] is displayed in the [Mode] group box in the [Acquire] panel.
2. Select the [XY] option button in the [Acquire] panel.