Olympus FLUOVIEW FV300, User Manual

Page: 164 / 564

APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 5 0
Page
Save image.
(Section 2-3-1 in [OPERATION])
Configure the microscope and scan unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-3-2 in [OPERATION])
Set the objective magnification.
(Section 2-2-1-3-1 in [OPERATION])
Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-3-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-3-4 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-3-6 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-3-5 in [OPERATION])
Set the observation range.
(Section 2-2-1-3-8 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-3-10 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-3-11 in [OPERATION])
Set the observation mode.
(Section 2-2-2-6-3 in [OPERATION])
Acquire image.
(Section 2-2-2-6-6 in [OPERATION])
Set the range of the multiple
sections to be observed (the Z-
direction scanning range).
(Section 2-2-2-6-1 in [OPERATION])
Set the numbers of Z-direction steps
and acquired image slices.
(Section 2-2-2-6-2 in [OPERATION])
Adjust the Z-position to observe the
desired multiple sections.
(Section 2-2-1-3-7 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Set the interval time.
(Section 2-2-2-6-4 in [OPERATION])
Set the number of scans.
(Section 2-2-2-6-5 in [OPERATION])
If the image
does not
become
clean
If the image
becomes
clean
Re-adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])