Olympus FLUOVIEW FV300, User Manual

Page: 82 / 564

Getting Started FLUOVIEW/
Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV
. 1 - 4 4
Page
1-2-11 Selecting the Laser Line Filters (Combination Using the Kr/Ar Laser)
Select the laser line filters using the laser line filter turret.
Reference Examples
Dye Laser Line Filters
FITC
Lucifer Yellow, etc.
488
FITC+TRITC
FITC+PI, etc.
568 568 568
+ or + or +
488 488 488
AT25 AT6
PI
TRITC, etc.
568
(See item (5), “Kr/Ar laser line filter switching” in section 1-1-1, “Scan Unit” in this
volume.)
1-2-12 Setting the Observation Condition
1-2-12-1 Setting the Objective Magnification
From the drop-down list on the center of the [Acquire] panel, select the objective being
used with the microscope.
If the magnification of the objective in use and that set here do not
match, the measurement results will be inaccurate.
1-2-12-2 Setting the Zoom Ratio to 1X
From the panel page tabs shown on the bottom right of the [Acquire] panel, select the
[Settings] sub-panel. Then use the [Zoom] scale to set the zoom ratio to “X1”.
NOTE