Olympus FLUOVIEW FV300, User Manual

Page: 158 / 564

APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 4 4
Page
2 Setting the numbers of s teps and acquired image slices
1. Set the number of steps using the < > or < > button in the [Step Size] text box.
The number of steps shown in the [Step Size] text box has been calculated
by the system so that the depth scale of the acquired image is identical to
the horizontal scale.
The number of acquired images shown in the [Slices] text box can also be
input from the keyboard.
After setting [Start Z] (Z-direction scan start position) and [Stop Z] (Z-
direction scanning stop position), input the desired number of images in the
[Slices] text box. This automatically sets [Step Size] (number of steps).
3 Setting the observation mode
1. Click the [Surface] option button in the [Mode] group box in the [Acquire] panel.
2. In the [Acquire] panel, select the XZT observation mode option button.
4 Acquiring image
1. Click the <XYZ> button in the [Acquire] panel.
The acquired image will be displayed in the [Live] panel.
While acquiring an image in the XYZ observation mode, clicking the <STOP SCAN>
button changes the buttons at the upper part of the [Acquire] panel as shown below. The
<Resume> button restarts image acquisition at the frame next to the frame where the
acquisition is suspended.
TIP
TIP
[Step Size] text box
[Slices] text box
<Resume> button
Restarts image acquisition at
the frame next to the frame
where the acquisition is
suspended.
<Series Done> button
Determines the acquired
images. Once this button is
clicked, it is not possible to
append an image.