Olympus FLUOVIEW FV300, User Manual

Page: 192 / 564

APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 7 8
Page
3. In the [Mode] group box in the [Acquire] panel, click the [Surface XY-Norm] option
button, select <Sequen> from the list below it and select the scan mode to be used
from the displayed icons.
Do not select <Line Aq> in the [Mode] group box in the [Acquire] panel
because the virtual channels are not compatible with Line Sequential
Scan.
If the observation mode is set to Sequential Scan, the [Dyes] sub-panel in
the [Acquire] panel cannot be used.
4. Using the < > and < > buttons in [Group], set the group according to the
reagent in use. This operation is not required if the current setting needs not be
changed. (Example: CH1 (FITC) and CH2 (PI) are set to Group 1.)
5. Right-click the mouse on the [Ch] group box on the upper part of the [Acquire]
panel. When a pop-up menu appears, select the channel to be added.
NOTE
TIP
Click to increase the acquired group No.
Click to decrease the acquired group No.
Shows the group to be acquired.
Select the channel to
which you want to add
a virtual channel.