Olympus FLUOVIEW FV300, User Manual

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Getting Started FLUOVIEW/
Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV
. 1 - 3 6
Page
1-2-4-4 Combination with Inverted Microscope (IX70)
1. Turn the light path selector (1) to
SP
.
2. Set the intermediate magnification knob (7) to 1X. (The 1.5X position cannot be
used.)
3. Rotate the cube turret of the reflected light fluorescence unit to .
4. When the IX-AN analyzer (3) is in use, disengage it from the light path by
setting the switch to the pulled-out position.
5. When only fluorescence observation is required, disengage the U-DICT transmitted
DIC slider (4) by setting the switch to the pulled-out position. When transmitted light
differential interference observation or simultaneous fluore transmitted
light differential interference observation is required, engage the U-DICT and the
optimum transmitted light DIC slider in the light path by operating the universal
condenser (6). With simultaneous fluore transmitted light differential
interference observation, leaving the U-DICT engaged in the light path will degrade
the fluorescence image resolution somewhat.
6. For transmitted light observation, disengage the LBD filter from the light path and
engage the FR (frost) filter in the light path by operating the filter levers. If the FR
filter is disengaged from the light path, the image may be marred by stripe
interference.