Olympus FLUOVIEW FV300, User Manual

Page: 133 / 564

APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 1 9
Page
9 Adjusting the image brig htness
1. While observing the image in the [Live] panel, change the setting values of the
PMT voltage [PMT], Offset [Offset] and Gain [Gain] in the [Acquire] panel. The
functions of these parameters are as described below.
PMT Voltage
Increasing this value improves the sensitivity.
However, too high a sensitivity makes image noise noticeable.
If sufficient brightness cannot be obtained by setting the PMT voltage to
800 V, leave it as it is and increase Gain. This will usually provide a better
result than using a PMT voltage over 800 V.
Offset
Darkens the image at the ratio set during image acquisition.
(This value can be set independently from the Gain.)
Gain
Brightens the image at the ratio set during image acquisition.
(This value can be set independently from the Offset.)
For example...
When the acquired image is dark or the observation targets are hardly visible, increase
PMT voltage to improve the sensitivity. If the image resulting from this becomes too
bright, first try decreasing PMT voltage slightly. If the image background is still too
bright, increase Offset to darken the background. As this also reduces the brightness of
the observation target, increase Gain as required so that the observation target is
clearly visible.
Gamma Correction provides you more brightened image even if it was acquired with
darkness.
Refer to “2-5-2-2 LUT Graph Editing by Gamma Correction “ for details in this operation.
Clicking this field allows the value to
be changed on a large scale.
The ND value which are usually used
are displayed in green.
Clicking this button allows fine
adjustment of the value.
Clicking the <+> or <-> button or the
field displays the set value. The set
value can be varied by direct input
from the keyboard.