Olympus FLUOVIEW FV300, User Manual

Page: 558 / 564

TROUBLESHOOTING GUIDE
VI .
TROUBLESHOOTING
VI.
1 - 2
Page
Phenomenon Cause Treatment Manual Ref. Pages
1. Fluorescence image
cannot be observed The fluorescent dye does not
match the barrier filter. Select the barrier filter
matching the fluorescent
dye.
See 2-2-1-2, “Configuring
the Scan Unit” in
OPERATION
INSTRUCTIONS and follow
instructions of the
[Microscope Configuration]
window.
2-2-2-1,
OPERATION
INSTRUCTIONS
1-2-8 & 1-3-2,
OPERATION
INSTRUCTIONS
Focusing is not correct. Adjust focusing. 1-2-3, OPERATION
INSTRUCTIONS
The photomultiplier tube
voltage of the detector is low. Increase the photomultiplier
tube voltage using the [PMT]
scale in the [Acquire] panel. 2-2-1-3-9,
OPERATION
INSTRUCTIONS
The OFFSET value is too
high. Decrease OFFSET to an
optimum value. 2-2-1-3-9,
OPERATION
INSTRUCTIONS
The photomultiplier tube of
the channel set with the
detection mode setting slider
is not set. Check the [Ch1], [Ch2] or
[Ch3] check box in the
[Acquire] panel.
1-1-1 & 2-2-1-3-3,
OPERATION
INSTRUCTIONS
2. Transmitted image cannot
be observed.
The transmittance detection
channel is not selected. In the [Acquire] panel, check
the check box of the [Ch]
with which “Transmitted” is
displayed. 2-2-3-3,
OPERATION
INSTRUCTIONS
The transmitted light filter is
engaged in the light path of
the microscope. Disengage the filter from the
light path. 1-1-2 & 1-2-4,
OPERATION
INSTRUCTIONS
3. Image is disturbed. The installation location is
subject to large vibrations. Consult Olympus. -
Darken the room before
acquiring image. - External light from a
fluorescent lamp, etc. is
detected.
Ensure that the top cover of
the scan unit is closed tightly. 1-1-1, OPERATION
INSTRUCTIONS
4. Image is striped. The laser reflection light is
penetrating. The barrier filter selection is
erroneous. 1-2-8, OPERATION
INSTRUCTIONS
5. Image looks poor. The analyzer is engaged in
the light path. Disengage the analyzer from
the light path. 1-2-3 & 1-2-4,
OPERATION
INSTRUCTIONS
The scan speed is high. Decrease the scan speed to
an optimum speed or
integrate the image. 2-2-1-3-10 & 2-2-1-
5, OPERATION
INSTRUCTIONS