Olympus FLUOVIEW FV300, User Manual

Page: 181 / 564

APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 6 7
Page
2-2-4 Image Acquisition by Rotating It (Rotation Scan)
Rotation scan enables image acquisition with tilting the field of viewed.
Rotation Scan can not be used with Free Line Scan.
This function is available when the FV5-IO3 is in use.
If you do not use the FV5-IO3, the [Pan] group box does not display the
arrow buttons and angle.
When acquiring an image by rotating it, also see section 2-2-6, “Image
Acquisition by Magnifying the Rectangular Position (Zoom-In Scan)”.
1. Acquire an image in the XY observation mode. For the operating procedure, see
section 2-2-1, “Image Acquisition in XY Observation Mode”.
2. Set the image acquisition mode.
For details, see sections 2-2-1, “Image Acquisition in XY Observation Mode”, 2-2-2,
“Image Acquisition in Other Observation Modes”, 2-2-5, “Image Acquisition of Only
the Rectangular Position (Clip Scan)”, 2-2-6, “Image Acquisition by Magnifying the
Rectangular Position (Zoom-In Scan)”, 2-2-7, “High-Speed Image Acquisition” and
2-2-8, “Image acquisition to prevent crosstalk between fluorescence (Sequential
Scan)”.
3. Specify the image rotation angle using the rotation arrow buttons on both sides of
the circuit in the [Pan] group box n the [Acquire] panel.
4. Acquire the image by clicking the <XYZ>, <XYT> or <XYZT> button.
NOTE
NOTE
Dragging the blue handle
along the circuit allows the
acquisition area to be tilted.
Assuming that the angle at
the top of the circle is 90°, tilts
the acquisition area by 1° per
step in the counterclockwise
direction.
Assuming that the angle at
the top of the circle is 90°, tilts
the acquisition area by 1° per
step in the counterclockwise
direction.
Displays the image rotation
angle. The angle can also be
specified by direct entry.
NOTE