Olympus FLUOVIEW FV300, User Manual

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Getting Started FLUOVIEW/
Outline of LSM Observation Procedures
IV.
OPERATION INSTRUCTIONS
IV.
1 - 4 1
Page
Be sure to set the switch to the fully pulled-out or pushed-in position. If the optimum
setting cannot be identified, see section 1-3-2-3, “Configuring the Filters” in this volume
and follow instructions in the [Microscope Configuration] window.
The following table shows the possible combinations of the barrier filters and excitation
dichroic mirrors.
Combinations of Barrier Filters, Excitation Dichroic Mirrors, and Beam Splitter
(Example)
Laser Combination
Excitation
Dichroic Mirror
Beam Splitter Barrier Filter 1 Barrier Filter 2
Ar
488
(2 channels) (1) DM488 (1) Mirror
(2) SDM570
(3) SDM630 (1) BA510IF (1) BA565IF
Ar, HeNe(G)
488, 543
(2 channels) (1) DM488/543 (1) Mirror
(2) SDM570
(3) SDM630 (1) BA510IF
(2) BA530RIF (1) BA565IF
Ar, Kr
488, 568
(2 channels) (1) DM488/568 (1) Mirror
(2) SDM570
(3) SDM630 (1) BA510IF
(2) BA550RIF (1) BA585IF
Ar, HeNe(R)
488, 633
(2 channels) (1) DM488/568/633 (1) Mirror
(2) SDM570
(3) SDM630
(1) BA510IF
(2) BA550RIF (1) BA660IF
Ar, HeNe(G), HeNe(R)
488, 543, 633
(2 channels) (1) DM488/543/633 (1) Mirror
(2) SDM570
(3) SDM630
(1) BA510IF
(2) BA530RIF (1) BA560-600
(2) BA660IF
Ar, Kr, HeNe(R)
488, 568, 633
(2 channels) (1) DM488/568/633 (1) Mirror
(2) SDM570
(3) SDM630
(1) BA510IF
(2) BA550RIF (1) BA585-615
(2) BA660IF
Multiline Ar
458/488/515
(2 channels) (1) DM488
(2) DM458/515 (1) Mirror
(2) SDM570
(3) SDM630
(4) SDM515 (1) BA510IF
(2) BA480-495 (1) BA565IF
(2) BA535-565
Multiline Ar, HeNe(G)
458/488/515, 543
(2 channels) (1) DM488/543
(2) DM458/515
(3) BS20/80 (1) Mirror
(2) SDM570
(3) SDM630
(4) SDM515 (1) BA510IF
(2) BA530RIF
(3) BA480-495 (1) BA565IF
(2) BA535-565
Multiline Ar, Kr
458/488/515, 568
(2 channels) (1) DM488/568
(2) DM458/515
(3) BS20/80 (1) Mirror
(2) SDM570
(3) SDM630
(4) SDM515 (1) BA510IF
(2) BA550RIF
(3) BA480-495 (1) BA585IF
(2) BA535-565
Multiline Ar, HeNe(R)
458/488/515, 633
(2 channels) (1) DM488/568/633
(2) DM458/515 (1) Mirror
(2) SDM570
(3) SDM630
(4) SDM515 (1) BA510IF
(2) BA550RIF
(3) BA480-495 (1) BA660IF
(2) BA535-565
Multiline Ar, HeNe(G),
HeNe(R)
458/488/515, 543, 633
(3 channels) (1) DM488/543/633
(2) DM458/515
(3) BS20/80 (1) Mirror
(2) SDM570
(3) SDM630
(4) SDM515 (1) BA510IF
(2) BA530RIF
(3) BA480-495 (1) BA560-600
(2) BA660IF
(3) BA535-565
Multiline Ar, Kr,
HeNe(R)
458/488/515, 568, 633
(3 channels) (1) DM488/568/633
(2) DM458/515
(3) BS20/80 (1) Mirror
(2) SDM570
(3) SDM630
(4) SDM515 (1) BA510IF
(2) BA550RIF
(3) BA480-495 (1) BA585-615
(2) BA660IF
(3) BA535-565