Olympus FLUOVIEW FV300, User Manual

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Software Setup/
Adding the dyeing method
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MAINTENANCE
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3. Display the [Fluorescent Dyes/Colors] panel at the front position in the [FLUOVIEW
Setup] dialog box.
4. Double-click the “Double click here to make a new Dye” in the [Dyes on your
Samples] list.
The [Edit Dye] dialog box as shown below appears.
5. Enter the name of the dyeing method into the [Name of the Dye] text box (e,g,
CFP).
6. Select the laser to use using the [Laser Type] drop-down list (e.g. HeCd).
As the laser to use, helium-cadmium laser should be set in advance. When
“HeCd” is not displayed, check the [HeCd] check box in the [Laser
Equipment] panel.
7. Enter the value of the excitation wavelength into the [WaveLength 1] text box in the
[Excitation Wave Length] group box (e.g. 442).
Double-click here to add
the dyeing method.
[[Name of the Dye] text box
Enter the name of the dyeing
method.
[Laser Type] drop-down list
Select the laser to use.
[Emission Wave Length]
group box
Enter the emission
wavelength.
In case of the 1-wavelength
and 2-photometry, enter the
value into the [[WaveLength2]
text box too.
[Excitation Wave Length]
group box
Enter the excitation
wavelength. In case of the 2-
wavelength and 1-
photometry, enter the value
into the [[WaveLength2] text
box
TIP