Olympus FLUOVIEW FV300, User Manual

Page: 194 / 564

APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 8 0
Page
6. Set the laser and filter types condition.
Select <SU Control> button of the [Settings] sub-panel in the [Acquire] panel to
display the [Optical System Configuration] window.
In the [Optical System Configuration] window, set the dyeing method of virtual
channel, then set the displayed optimum filter, etc. of the window in the scanning
unit.
For details on the [Optical System Configuration] window, see section 1-3-2-3,
“Configuring the Filters”.
(Example: Set the group No. to [2] in the [Group #] drop-down list of [For Seq.
Scan]. Then open the [Group] drop-down list of vCh (2) in the [Virtual
Channel] group box and select [2].
In the [Dye] drop-down list of vCH (2), select [CY5].
Displayed each optimum filters, etc. in [Excitation DM], [Beam Splitter],
[Barrier Filter] and [Laser Unit] group box.
After completing the setup, select the <Close> button to close window.
[Virtual Channels] group box
Show the virtual channels.
Click a channel checkbox to check it makes the
virtual channel available. Select the dyeing method
from the drop-down list and select the group No. in
the [Group] drop-down list.
[Assign Laser by Dye] check
box
When checked, settings of filter
and laser will be automatically
done according to selection of dye
method.
[Confirm the SU setting every
scan for Group] check box
When check is removed, dialog
does not appear each time
group is changed during scan;
and it is possible to execute
scanning consecutively.