Olympus FLUOVIEW FV300, User Manual

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APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
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Page
2. While observing the image in the [Live] panel, locate the upper edge of the range
to be observed by moving down the stage using the <Z stage coarse adjustment>
and <Z stage fine adjustment> buttons in the [Z Stage] sub-panel.
When using the FLUOVIEW system with an inverted microscope, locate the bottom
edge of the range to be observed by moving down the revolving nosepiece using
the <Z stage coarse adjustment> and <Z stage fine adjustment> buttons.
3. When the upper edge position is located, click the <Set> button. The [Start Z] text
box will show the scan start position of the range of the multiple sections to be
observed (Z-direction scanning range).
4. While observing the image in the [Live] panel, locate the bottom edge of the range
to be observed by moving up the stage using the <Z stage coarse adjustment> and
<Z stage fine adjustment> buttons in the [Z Stage] sub-panel.
When using the FLUOVIEW system with an inverted microscope, locate the upper
edge of the range to be observed by moving up the revolving nosepiece using the
<Z stage coarse adjustment> and <Z stage fine adjustment> buttons.
5. When the position is located, click the <Set> button. The [Stop Z] text box will
show the scanning stop position of the range of the multiple sections to be
observed (Z-direction scanning range).
2 Setting the observation mode
1. Click the [Depth] option button in the [Mode] group box.
2. In the [Acquire] panel, select the XZT observation mode option button.
3 Setting the observation line
1. A line is displayed on the image in the [Live] panel. Place the mouse pointer arrow
on the line and drag it to the position you want to observe.