Olympus FLUOVIEW FV300, User Manual

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Getting Started FLUOVIEW/
Basic Operations
IV.
OPERATION INSTRUCTIONS
IV .
1 - 1
Page
1 Getting St arted FLUOVIEW
1-1 Basic Operations
1-1-1 Scan Unit
(1) Confocal aperture switching
The confocal aperture includes 5 positions, any of which can be
selected by rotating the turret.
Rotate the CONFOCAL APERTURE turret to select the optimum
confocal aperture for the objective in use.
Select the optimum confocal aperture number for the objective being
displayed in the control panel displayed by the computer ([Acquire]
panel).
When the acquired image is bright enough and noise is not
noticeable even when the HV of photomultiplier is increased, an
even better image may be obtained by selecting a smaller confocal
aperture size by rotating the CONFOCAL APERTURE knob.
The actual sizes of the confocal aperture positions are as listed
below.
(1) CONFOCAL APERTURE turret
(2) DETECTION MODE slider
(3) BARRIER FILTERS
slider
(4) Excitation DM
selector knob