Olympus FLUOVIEW FV300, User Manual

Page: 155 / 564

APPLIED OPERATIONS
/Image Acquisition
IV.
OPERATION INSTRUCTIONS
IV .
2 - 4 1
Page
2-2-2-4 XYZ Observation Mode
The description in this section will be focused on the image acquisition operations in the XYZ
observation mode that are not used in the XY observation modes (which are the operations
enclosed in in the chart on the next page). For other operations, see section 2-2-1,
“Image Acquisition in XY Observation Mode”. The details of each operation will be described in
the subsequent sections.
Save image.
(Section 2-3-1 in [OPERATION])
Configure the microscope and scan unit.
(Sections 2-2-1-1 & 2-2-1-2 in [OPERATION])
Set the objective magnification.
(Section 2-2-1-3-1 in [OPERATION])
Set the dyeing method.
(Section 2-2-1-1 in [OPERATION])
Set the channel to be acquired.
(Section 2-2-1-3-3 in [OPERATION])
Set the highest scan speed.
(Section 2-2-1-3-4 in [OPERATION])
Perform repeated scanning.
(Section 2-2-1-3-6 in [OPERATION])
Set the XY observation mode.
(Section 2-2-1-3-5 in [OPERATION])
Set the observation range.
(Section 2-2-1-3-8 in [OPERATION])
Set a lower scan speed.
(Section 2-2-1-3-10 in [OPERATION])
Stop repeated scanning.
(Section 2-2-1-3-11 in [OPERATION])
Set the observation mode.
(Section 2-2-2-4-3 in [OPERATION])
Acquire image.
(Section 2-2-2-4-4 in [OPERATION])
Set the numbers of Z-direction steps
and acquired image slices.
(Section 2-2-2-4-2 in [OPERATION])
Adjust the Z-position to observe the
desired multiple sections.
(Section 2-2-1-3-7 in [OPERATION])
Adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Set the zoom ratio to 1X.
(Section 2-2-1-3-2 in [OPERATION])
If the image
does not
become clean If the image
becomes
clean
Re-adjust the image brightness.
(Section 2-2-1-3-9 in [OPERATION])
Set the range of the multiple sections to be
observed (the Z-direction scanning range).
(Section 2-2-2-4-1 in [OPERATION])