Olympus FLUOVIEW FV300, User Manual, Page: 29 / 564

CONTENTS
1 Getting Started FLUOVIEW 1-1
1-1 Basic Operations .............................................................................1-1
1-1-1 Scan Unit .....................................................................................................1-1
1-1-2 Microscope ..................................................................................................1-8
1-1-3 General Mouse Operation Procedures.......................................................1-17
1-1-4 Names of Major Panel and Window Controls and Their Functions.............1-18
1-2 Outline of LSM Observation Procedures.....................................1-20
1-2-1 Turning Power On......................................................................................1-22
1-2-2 Starting the Software .................................................................................1-26
1-2-3 Focusing on the Specimen.........................................................................1-28
1-2-3-1 Combination with BX........................................................................................1-28
1-2-3-2 Combination with IX .........................................................................................1-29
1-2-4 Setting the LSM Light Path ........................................................................1-31
1-2-4-1 Combination with Upright Microscope (BX) .....................................................1-31
1-2-4-2 Combination with Erected Microscope(AX) .....................................................1-35
1-2-4-3 Combination with Inverted Microscope (IX81) .................................................1-36
1-2-4-4 Combination with Inverted Microscope (IX70) .................................................1-38
1-2-5 Setting the Dyeing Methods .......................................................................1-40
1-2-6 Selecting the CONFOCAL APERTURE .....................................................1-42
1-2-7 Selecting the DETECTION MODE .............................................................1-42
1-2-8 Setting the Barrier filters ............................................................................1-42
1-2-9 Setting the Excitation DM...........................................................................1-45
1-2-10 Setting the ND Filters of the Laser Combiner...........................................1-45
1-2-11 Selecting the Laser Line Filters (Combination Using the Kr/Ar Laser)......1-46
1-2-12 Setting the Observation Condition............................................................1-46
1-2-12-1 Setting the Objective Magnification................................................................1-46
1-2-12-2 Setting the Zoom Ratio to 1X.........................................................................1-46
1-2-12-3 Setting the Channels......................................................................................1-47
1-2-12-4 Setting the Highest Scan Speed ....................................................................1-48
1-2-12-5 Setting the XY Observation Mode..................................................................1-49
1-2-12-6 Repeated Scanning Operation.......................................................................1-49
1-2-12-7 Setting the Multiple sections to be Observed.................................................1-50
1-2-12-8 Setting the Area to be Observed....................................................................1-51