Olympus FLUOVIEW FV300, User Manual

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Getting Started FLUOVIEW/
Basic Operations
IV.
OPERATION INSTRUCTIONS
IV .
1 - 1 2
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(1) Light path selector knob
Select the light path between direct observation and laser microscopy.
x
Set to the pushed-in position for direct observation.
x
Set to the pulled-out position for laser microscopy.
(2) Universal vertical illuminator mirror cube housing
The turret for the cube for reflected light observation can be switched with the hand
switch for transmitted/reflected light.
x
Engage the desired cube in the light path for direct fluorescence observation.
x
For laser microscopy or for direct transmitted light observation, push BF
button in cube selector buttons in Hand switch for transmitted/reflected light.
(This sets the cube turret so that no cube is engaged.)
(3) Analyzer AX-AN(optional)
Polarizing plate for use in differential interference observation and polarized light
observation.
x
Set to the pushed-in position to engage the AX-AN in the light path for direct
transmitted light differential interference observation or transmitted polarized
light observation.
x
Set to the pulled-out position to disengage the U-MDICT3 for laser
microscopy. Engaging the AX-AN in the light path allows improvement of the
acquired image quality.
(4) Transmitted light DIC slider U-DICR(optional)
This is the prism for use in differential interference observation.
x
Engage the transmitted light DIC Slider in the light path for laser differential
interference observation or direct transmitted light differential interference
observation.
Leaving the transmitted light DIC slider engaged during laser fluorescence
observation will degrade the image quality somewhat. We recommend
disengaging the transmitted light DIC slider from the light path when simple
laser fluorescence observation is required.