Olympus FLUOVIEW FV300, User Manual

Page: 345 / 564

APPLIED OPERATIONS
/Image Processing
IV.
OPERATION INSTRUCTIONS
IV .
2 - 2 3 1
Page
2-6-1-4 Correcting DIC Level Irregularities
DIC level irregularities refers to uneven brightness of the image background which may
be observed when a transmitted image is acquired in IDC observation.
The DIC level irregularities can be corrected to make the image easier to view.
If fluorescence observation is used, note that the correction is possible only with the
transmitted images.
1. Display the [Filters] sub-panel in the [Process] panel.
Fig. 2-86 [Filters] Sub-panel
2. Enter the appropriate correction factor for the image background in the [kg] text
box.
The correction factor can be set between 0 and 1 in steps of 0.1.
The standard factor is 1. If the effect is extreme, try using 0.5.
<DIC leveling> button
Automatic correction of
irregularities of the image
background.
[kg] text box
Enter the correction factor
of the image background.
Irregularities in brightness are
observed across the diagonal
line of the background.
TIP