Olympus FLUOVIEW FV300, User Manual

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Getting Started FLUOVIEW/
Basic Operations
IV.
OPERATION INSTRUCTIONS
IV .
1 - 9
Page
(1) Light path selector knob
Select the light path between direct observation and laser microscopy.
x
Set to the pushed-in position for direct observation.
x
Set to the pulled-out position for laser microscopy.
(2) Cube turret
Select the fluorescence observation tube by rotating the turret.
x
Engage the desired cube in the light path for direct fluorescence observation.
x
For laser microscopy or for direct transmitted light observation, rotate the
turret to page tab . (This sets the cube turret so that no cube is
engaged.)
(3) Analyzer U-MDICT3
Polarizing plate for use in differential interference observation and polarized light
observation.
x
Rotate the turret to engage the U-MDICT3 in the light path for direct
transmitted light differential interference observation or transmitted polarized
light observation.
x
For laser microscope, always disengage the U-MDICT3. Engaging the U-
MDICT3 in the light path degrades the image quality.
(4) Transmitted light DIC U-DICTS/WI-DICTHRA (when using BX61WI) (optional)
This is the prism for use in differential interference observation.
x
Engage the transmitted light DIC in the light path for laser differential
interference observation or direct transmitted light differential interference
observation.
Leaving the transmitted light DIC engaged during laser fluorescence
observation will degrade the image quality somewhat. We recommend
disengaging the transmitted light DIC from the light path when simple laser
fluorescence observation is required.
With transmitted light differential interference observation
using an immersion objective, set the microscope’s field
diaphragm so that it circumscribes the field of view. Otherwise
the contrast may degrade. (This applies to both direct
observation and laser differential interference observation.)
NOTE