Dionex As, Operator'S Manual

Page: 85 / 356

3 • Operation and Maintenance
Doc. 065051-03 1/08
73
3.6.7 Normal, Partial-Loop Injections
For a partial-loop injection, the AS draws the volume of sample to be
injected from the sample vial plus two times the
cut volume. The cut
volume specifies a volume of sample to be “cut” (or omitted) from each
side of the sample during the injection process.
See Section 3.6.3 for
details. The middle portion of the sample is positioned in the loop and
injected (see
Figure 3-20 ).
Figure 3-20. Partial-Loop Injection Sequence
Sample
Syringe
1. Draw air
3. Draw air again to
isolate the sample.
KEY
Eluent
Sample
6. Deliver sample
to
the loop
(inject volume)
LOAD
Pump Column
INJECT
Pump Column
INJECT
Pump Column
Waste Waste Waste
Flush
Air
4. Draw flush
fluid to position
sample in loop.
2. Draw sample
(injection volume
+ 2X cut volume)
5. Prepare to load
sample (flush fluid +
air cut
volume #1 is used)
7. Inject sample
onto column. Empty
sample syringe (cut
volume #2 + air bubble)
L
P C
L
S
W
L
P C
L
S W
L
P C
L
S W
Sample
Air
Flush