Dionex As, Operator'S Manual

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AS Autosampler Operator’s Manual
238
Doc. 065051-03 1/08
Prep Operation Description
DELAY Specifies for how long the AS waits (0.0 to 999.9 minutes)
before proceeding to the next step in the sample prep phase
(default=0.0).
NEEDLE The height of the needle above the bottom of the vial, from 0 to
46 mm (default=2 mm). The needle height is used for all pipet,
dispense, and mix operations until a new height is specified.
NOTE For 0.3 mL and 1.5 mL vials, do not set
the needle height greater than 2 mm.
This ensures that the vent in the
sampling needle is positioned correctly
inside the vial.
PIPET Moves a specified volume of sample from one vial to another.
The sample syringe is always used for pipetting.
μ
L:
Pipet volume: from 1.0 to 99.9
μ
L (in
0.1
μ
L increments) or 100 to 1,000
μ
L (in 1
μ
L
increments) (default=20
μ
L).
#:
Source vial position (the vial from which to
pick up the specified volume): (default=
S+1
).
to #:
Destination vial position (the vial to receive
the volume of sample): (default=
SMP
).
MIX Mixes the vial contents by repeatedly drawing and expelling a
volume of sample. Mixing is done with the prep syringe, if it is
installed; otherwise, the sample syringe is used.
#
: Mix vial position (the position of the vial to be mixed):
(default =
SMP
).
X
: Mix cycles (the number of times to repeat the
mixing operation): from 1 to 99 (default=10).
u
L
Mix volume (the volume to be drawn and expelled):
from 1.0 to 9,999
μ
L. The default is 500
μ
L if the prep
syringe is installed; otherwise, it is 250
μ
L. The mix
volume must be equal to or less than the syringe
volume.