Dionex As, Operator'S Manual

Page: 172 / 356

AS Autosampler Operator’s Manual
160
Doc. 065051-03 1/08
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Step 2: Clicking Setup empties the needle seal line and the diverter line.
•
Step 3: Enter an approximate volume using the following formula:
0.8
μ
L/in (2
μ
L/in) for the diverter line plus the volume of the needle seal
line (which is written on a label on the tubing and inside the AS front
door). Then, click
Estimate . The AS dispenses the volume entered, which
nearly fills the diverter and needle seal lines (minus a few microliters).
NOTE The value used for approximating the diverter line
volume (0.8
μ
L/cm, 2
μ
L/in) assumes a tubing ID of
0.330 mm (0.130 in). For volume per length values for
other tubing IDs, see
Section 3.8 .
•
Step 4: After clicking +1 uL
,
wait until the sound of the syringe motor
stops before clicking +1 uL again.
Continue clicking +1 uL until a tiny drop of liquid appears at the end of
the diverter line. If you go too far and more than a single small drop of
liquid appears, click -
5 uL . The AS will empty the line and then fill the
line with 5 μ L less than was cleared. You can then begin clicking +1 uL
again.
•
Step 5: After clicking Test , verify that a drop of liquid appears at the end
of the diverter line. If no drop appears or if the drop is a different size than
previously observed, click -
5 uL and repeat the procedure.
•
Step 6: After clicking Done , the new calibration value is displayed on the
panel. Copy this value to the label inside the AS front door.
•
Calibrate System 2: Select Diverter Valve Position_2 and repeat the
calibration procedure for system 2.
•
Finishing: Reconnect the diverter lines to the injection valves. Tighten
the fittings fingertight and then tighten an additional one-quarter turn
using the 5/16-in wrench.