Dionex As, Operator'S Manual

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2 • Description
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2.7.8 Operating Events During Flushing and Priming
The AS automatically flushes the inject port and washes the outside of the
needle at the following times:
•
At the beginning and end of a Chromeleon or Chromeleon Xpress
sequence or a front panel schedule
•
Before each injection, while the schedule or sequence is running
Flushing can also be initiated manually when the AS is idle, or as a step in
the sample prep phase of a Chromeleon or Chromeleon Xpress program
or front panel method.
Manual priming of the flush and reagent lines must be initiated after
refilling a reservoir, or after the AS has been idle for a period of time. See
Section B.3.13 for instructions on priming the liquid lines.
Flushing
The AS performs the following operations during flushing:
•
The sampling needle arm moves to the waste port and expels any
fluid from the needle.
•
The sampling needle arm moves to the inject port and delivers a
volume of fluid for flushing the inject port.
•
The sampling needle arm moves to the flush port and delivers a
volume of flush fluid. This washes the outside of the needle.
The flush reservoir and sample syringe are always used to perform the
flush.
The needle is washed with a factory-set volume. Select the volume of
liquid for flushing or priming on the Chromeleon or Chromeleon Xpress
Control panel (see
Figure 2-13 ) or on the
FLUSH/PRIME
screen (see
Section C.7 ). If you specify a flush volume that is greater than the sample
syringe volume, the
AS
performs multiple syringe strokes until the
volume is reached.
If you are using Chromeleon or Chromeleon Xpress to control the
autosampler, you can specify separate syringe speeds for aspirating and
dispensing liquid during flushing or priming. Specify the speeds on the
Control panel or in the program (see
Section 3.7 ).