Dionex As, Operator'S Manual

Page: 298 / 356

AS Autosampler Operator’s Manual
286
Doc. 065051-03 1/08
Pipetting Liquid from One Vial to Another
The
PIPET
operation uses the sample syringe to transfer liquid from a
source vial to a destination vial. After pipetting, mix the destination vial
before sampling from it. Be sure to specify enough mix cycles to
completely mix the vial contents (see “Mixing a Vial” below).
NOTE Unlike the
DILUTE
operation, the
PIPET
operation does
not include an automatic mixing step. If the sample
preparation option is installed, use the
DILUTE
operation, not
PIPET,
for dilutions (see “Diluting” on
page 288 ).
Mixing a Vial
The
AS
mixes the vial by repeatedly drawing up and expelling a volume
of the contents. The prep syringe is used if the sample preparation option
is installed; otherwise, the sample syringe is used. When specifying the
volume, enter a value that is less than or equal to the volume of the
syringe. If the volume is greater than the syringe volume, an error
message occurs and the syringe volume is used.
20.0 uL to #S+1 #SMP PIPET
Source Vial Destination Vial Volume to Pipet
Volume to Pipet
1 to 100
1 to 49
SMP, S+1, ..., S+9 (aluminum tray)
(plastic tray)
1 to 100
SMP, S+1, ..., S+9, WST
1 to 49
1.0 to 99.9 L
100 to 1000 L
μ
μ
Source Vial
Destination Vial
MIX
times 10 uL 250 #SMP
Vial to Mix
Vial to Mix
1 to 100 (aluminum tray)
1 to 49 (plastic tray)
SMP, S+1, ..., S+9
1 to 99 1 to 9999 L
μ
Number of
Mix Cycles
Cycles
Volume to
Aspirate and
Expel per Cycle
Volume