Dionex As, Operator'S Manual

Page: 166 / 356

AS Autosampler Operator’s Manual
154
Doc. 065051-03 1/08
Step 2: After clicking Test , adjust the
X-position
and/or
Y-position
values until
the needle is centered in the inject port.
Step 3:
•
Select the syringe speed and diverter valve position (if installed) that will
provide the maximum backpressure delivered to the needle seal during
use. This backpressure must not exceed 690 kPa (100 psi). Refer to
Section 3.8 for guidelines on selecting syringe speeds.
•
After selecting the syringe speed and the diverter valve position (if
installed), click
Test . The AS dispenses 100
μ
L of fluid into the port.
•
Monitor the top of the inject port for fluid. If fluid appears, wipe up the
fluid immediately. Then, increase the needle’s
z-axis height and click
Move to move the needle down. Note : Each digit in the z-axis height
value moves the needle 0.127 mm (0.005 in).
Step 4:
•
After clicking Check , the AS dispenses 500 μ L of fluid into the port. This
larger amount of fluid provides a more thorough check than the 100
μ
L
that is dispensed by clicking Test in Step 3.
•
After clicking Finish , the new calibration values are displayed on the
panel. Write these values on the label inside the AS front door.
5. If you are performing this procedure after replacing the needle seal assembly,
go on to calibrate the inject port volume (see Section 5.9 ).
Achten Sie beim Betrieb mit geöffneter Tür auf unerwartete
Bewegungen des Probenarms.
Increase the z-axis height value only until no more leaks occur. If the
z-axis height is too large, the needle will hit the bottom of the port
during operation and can damage the needle or port.