Dionex As, Operator'S Manual

Page: 83 / 356

3 • Operation and Maintenance
Doc. 065051-03 1/08
71
Setting Up a Full-Loop Injection
1. On the
SYSTEM PARAMETERS
screen (see Section C.6.5 ), select
NORMAL
in the
SAMPLE MODE
field.
2. In the Chromeleon or Chromeleon Xpress sequence (see Figure 3-19 )
or in the front panel schedule (see Section D.3 ), enter an injection
volume equal to or greater than the volume of the loop installed on
the injection valve.
Full-Loop Injection Notes
•
Entering an injection volume equal to or greater than the sample loop
size sets up the AS for a full-loop injection. The actual volume of
sample injected equals the volume of the sample loop installed.
•
If the entered injection volume is more than four times the loop size,
the entire injection volume is delivered during loading of the loop.
For example, if a 100
μ
L loop is installed and the injection volume is
600 μ L, then 350 μ L is flushed through the loop, 100 μ L is loaded
into the loop, and 150
μ
L remains before the loop.
Figure 3-19. Sequence Wizard: Specifying the Injection Volume