Dionex As, Operator'S Manual

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AS Autosampler Operator’s Manual
98
Doc. 065051-03 1/08
Example Backpressure Calculations for a Dual ICS-3000 System
The example tubing IDs and lengths for a dual ICS-3000 system shown in
Figure 3-35 will be used to calculate the total backpressure produced for each
system. See the following tables for details.
Prep Syringe Size Prep Syringe Speed Flow Rate (mL/min)
250, 500, and 1,000
μ
L 1 0.03
2 0.09
3 0.24
4 0.60
5 1.20
2.5, 5, and 10 mL
a
1 1.80
2 2.40
3 4.80
4 7.20
5 15.00
Table 3-12. Flow Rates Provided by the Prep Syringe
a. In general, if you are using a 5 or 10 mL syringe, select a syringe speed of 1 or 2.
Figure 3-35. Example Tubing IDs and Lengths for a Dual ICS-3000 System
1
2
P P
P
Needle Seal Line
Diverter Valve
Diverter Line #1
W
L
P
C
L
S
Inject Valve #1
Inject Valve #2
W
L P
C
L
S
Diverter Line #2
0.010-in ID, 46 cm
S
0.013-in ID, 38 cm
0.013-in ID, 64 cm
Inject Port
Needle
0.010-in ID
12 cm
25 µ L Loop
5 µ L Loop
0.020-in ID
15 cm