Dionex As Operator'S Manual Download Page 345

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Index

Doc. 065051-03 1/08

Index-7

Selecting, 60 – 65
Sequential, 60, 86 – 89
Sequential concentrate, 90 – 9 1
Simultaneous, 60, 78 – 7 9
Summary of, 60

Injection valve

Connections, 191
Controlling directly, 234
Controlling in a Chromeleon/Chromeleon

Xpress program, 72, 80 – 81, 89

Controlling in a front panel method, 296
Flow schematic, 21
Monitoring usage, 31, 129
Plumbing, 219
Rebuilding, 170
Specifications, 176

Injection volume, 246

Maximum allowed, 68 – 69
Ranges allowed per syringe size and

sample mode, 58

Simultaneous injection mode, 246
Specifying in a front panel schedule, 246,

298, 30 2

Specifying in Chromeleon/Chromeleon

Xpress, 59, 64

Injections

Controlling the time between, 36
Controlling the valve, 296
Running a Chromeleon/Chromeleon

Xpress sequence, 54

Running a front panel schedule, 274
Sample volumes used, 66 – 68
Selecting the type of, 60 – 65
Specifications, 176
Valve flow description, 21

Injections per vial

Specifying in a schedule, 246, 3 02
Zero, 299

Inserting lines in a method, 10
Inserting steps in a schedule, 10, 301
Installation, 179

AS with ICS-3000 system, 204
Connecting a second AS, 200
Diverter valve connections, 197

Dual AS connections, 200
Injection valve connections, 191
Injection valve connections for

simultaneous injection, 193

Module setup, 208
PC connections, 203 – 205
Power cord, 206
Priming, 214 – 217
Reagent reservoir connections, 190
Sample mode setup, 209
Sample Preparation option, 190
Simultaneous injection connections, 193
Syringe cable connections, 189
Syringe liquid line connections, 187
TTL/relay connections, 318
Unpacking, 180
USB connections, 203 – 205

Installation checklist, 186
Installed Options screen, 45, 207, 2 47
Installing the drip tray, 146
Insulated aluminum vial tray, 19
Invalid leak sensor reading, 119
Inverted edge TTL input control, 259, 320
Inverted pulse TTL input control, 259, 3 20
Isopropyl alcohol, 134

Key sound

Turning on and off, 248

Keyboard Test screen, 271
Keypad, 9
Keypad buttons, 10

See also

Buttons

Large-loop injection, 60

Sequence of events, 72
Volume injected, 66 – 67

LCD, 8 – 9, 12
Leak detected in tray/syringe area, 118

Summary of Contents for As

Page 1: ...AS Autosampler Operator s Manual Document No 065051 Revision 03 January 2008...

Page 2: ...ONEX CORPORATION DOES NOT WARRANT GUARANTEE OR MAKE ANY EXPRESS OR IMPLIED REPRESENTATIONS REGARDING THE USE OR THE RESULTS OF THE USE OF THIS PUBLICATION IN TERMS OF CORRECTNESS ACCURACY RELIABILITY...

Page 3: ...es 7 2 2 Front Panel Features 9 2 2 1 Keypad Button Functions 10 2 2 2 Display Screens 12 2 3 Autosampler Compartment 14 2 3 1 Syringe and Reservoir Organizer 15 2 3 2 Sampling Needle Arm 17 2 3 3 Sam...

Page 4: ...redictive Performance 31 2 7 Theory of Operation 33 2 7 1 Understanding the Wait Operation 33 2 7 2 Overlapping Sample Preparation 34 2 7 3 Running Samples Without Overlap 35 2 7 4 Operating the AS as...

Page 5: ...sitions 57 3 5 Specifying Injection Volumes 58 3 6 Sample Injection Types 60 3 6 1 Overview 60 3 6 2 Determining the Injection Type 62 3 6 3 Determining the Sample Loop Injection Type 63 3 6 4 Guideli...

Page 6: ...Daily 102 3 10 2 Periodically 103 3 10 3 Annually 103 3 10 4 Manually Flushing the Inject Port 103 3 11 System Shutdown 104 4 Troubleshooting 4 1 Error Messages 105 4 2 Liquid Leaks 124 4 2 1 Leaking...

Page 7: ...r 136 5 4 Replacing the Sampling Needle Assembly 136 5 4 1 Removing the Old Sampling Needle Assembly 138 5 4 2 Installing the New Sampling Needle Assembly 142 5 4 3 Resetting the Sampling Needle Assem...

Page 8: ...176 A 1 4 Injection 176 A 2 Sample Temperature Control Optional 177 B Installation B 1 Facility Requirements 179 B 2 Unpacking 180 B 3 Autosampler Installation 186 B 3 1 Before You Begin 186 B 3 2 Ins...

Page 9: ...es from the Syringe 218 B 4 Injection Valve Plumbing 219 B 5 Setting Up Chromeleon or Chromeleon Xpress for Simultaneous Injections 220 B 5 1 Assigning Unique Names to Devices 220 B 5 2 Creating Seque...

Page 10: ...n Screen 259 C 9 Diagnostic Menu 260 C 9 1 Power Up Screen 260 C 9 2 Diagnostic Tests Screen 261 C 9 3 XYZ Test Screen 262 C 9 4 Temperature Statistics Screen 263 C 9 5 Liquid Control Screen 264 C 9 6...

Page 11: ...d Events Steps 294 D 5 6 Editing a Method 297 D 5 7 Deleting a Method 297 D 5 8 Copying a Method 297 D 5 9 Example Method for Dilution 298 D 6 Creating Schedules from the AS Front Panel 298 D 6 1 Crea...

Page 12: ...1 08 E TTL and Relay Control E 1 TTL and Relay Connections 317 E 1 1 Connecting a TTL or Relay 318 E 1 2 Selecting TTL Input Control Types 319 E 2 Controlling TTL and Relay Outputs 321 E 2 1 Example T...

Page 13: ...ependent ion chromatography IC systems Sample is delivered equally to the two IC systems 50 of the volume to each This mode requires the simultaneous injection option In sequential mode a diverter val...

Page 14: ...uring sampling the tray remains stationary and a sampling needle arm moves from position to position Up to 99 injections can be taken from each vial or well Control The AS can be controlled locally fr...

Page 15: ...instructions for the AS and describes routine preventive maintenance procedures Chapter 4 Troubleshooting Lists problems and presents step by step procedures for how to isolate and eliminate the cause...

Page 16: ...ation which if not avoided may result in minor or moderate injury Also used to identify a situation or practice that may seriously damage the instrument but will not cause injury Indicates that the fu...

Page 17: ...suggest how to optimize instrument performance etc Bedeutet unmittelbare Gefahr Mi achtung kann zum Tod oder schwerwiegenden Verletzungen f hren Bedeutet eine m gliche Gef hrdung Mi achtung kann zum...

Page 18: ...cTUVus Mark safety labels and the CE Mark label on the AS indicate that the AS is in compliance with the following standards EN 61010 1 2001 safety CAN CSA C22 2 No 1010 1 92 A2 97 safety UL 61010C 1...

Page 19: ...Description 2 1 Overview of Features Figure 2 1 illustrates the main features of the AS Autosampler Figure 2 1 AS Operating Features Front Panel Sample and Prep Syringes Autosampler Compartment Power...

Page 20: ...le syringe the flush liquid reservoir and the optional prep syringe and reagent reservoirs See Section 2 3 1 for details Options Sample Preparation When this option is installed the AS can perform sam...

Page 21: ...ntial injection option a diverter valve is installed in the AS 2 2 Front Panel Features Figure 2 2 AS Front Panel Information is displayed on the LCD or screen To adjust the screen contrast use the kn...

Page 22: ...PLE PREP TIMED EVENTS or SCHEDULE screen and press Insert The new step is added above the cursor position See Appendix D for details about front panel operation Delete performs several functions Cance...

Page 23: ...g a cursor arrow button In fields that have predetermined numeric choices Select increases the value by one unit and Select decreases the value by one Holding down a Select button increases or decreas...

Page 24: ...us Screen NOTE TRAY temperature is displayed only when the sample temperature control option is installed Help Prompt Current vial or well Time since last injection Volume to be injected Current metho...

Page 25: ...cursor arrow button to position the cursor in the field to be edited Fields that are in reverse video blue letters on white background can be edited Other fields display information only 2 If the fiel...

Page 26: ...re 2 5 Autosampler Compartment Features Flush Waste and Inject Ports Prep Syringe Optional XYZ Sampling Needle Arm 3 4 7 Sample Tray for Vials or Well Plates Sample Syringe Reagent Reservoir Optional...

Page 27: ...awing and expelling the vial contents If the AS is equipped with the sample preparation option mixing is done with the sample prep syringe instead of the sample syringe Delivers sample to the injectio...

Page 28: ...d liquid extractions by adjusting the height of the needle in the vial The following sample prep syringe volumes are available The reagent reservoir connects to port A on the sample prep valve Additio...

Page 29: ...om of the vial or well can be adjusted allowing operations such as liquid liquid extraction You can set the needle height in a Chromeleon or Chromeleon Xpress program see Section 3 2 2 or in a front p...

Page 30: ...elow Figure 2 6 Sample Syringe with Sampling Needle Assembly Sampling Needle Assembly Used With Part Number PEEK Needle with 1 2 mL line Sample syringe volumes 1 mL or less 054271 PEEK Needle with 8 5...

Page 31: ...Vial Trays and Vials The following vial trays are available See the following section for well plate information The following vials are available Figure 2 7 Flush Waste and Inject Ports Tray Materia...

Page 32: ...ion Well Plate Tray Notes All well plate trays can be used with the sample temperature control option Each well plate tray also holds ten 1 5 mL vials The micro well plate tray can hold either type of...

Page 33: ...s from the AS inject port line through the valve and into the sample loop where it is held until injection Eluent flows from the pump through the valve and to the column bypassing the sample loop Exce...

Page 34: ...rammed set point to a minimum of 20 C below ambient and a maximum of 40 C above ambient The absolute range is 4 C to 60 C The sample temperature control option sits on the workbench below the autosamp...

Page 35: ...5 mL sampling needle assembly see Section 2 3 3 Full loop injections are required with this option See Section 3 6 9 for details about simultaneous injections 2 3 9 Sequential Injection Option When t...

Page 36: ...w larger sample loading volumes If the 5 or 10 mL sample syringe is installed an 8 5 mL sampling needle assembly must also be installed see Section 2 3 3 The AS is capable of delivering at a maximum p...

Page 37: ...hromeleon Xpress computer To enable computer control of the autosampler connect a USB cable between the USB receptacle on the AS rear panel and a USB port on the computer or USB hub See Section B 3 8...

Page 38: ...The fuse holder contains two 3 15 amp fast blow fuses P N 954745 For instructions on how to change the fuses see Section 5 18 The power cord plugs into the IEC 320 three prong receptacle The power sup...

Page 39: ...ect control you enter control commands and parameters on the AS screens and the AS executes the commands immediately In Schedule control you define a list of injections a schedule to be run in sequenc...

Page 40: ...run on each injection The action to take when a scheduled vial is missing You program save and edit schedules from the SCHEDULE screen see Figure 2 12 The AS can store up to nine schedules in memory...

Page 41: ...See Appendix D for details about front panel operation Some autosampler operations are not available from the AS front panel and can only be accessed from Chromeleon or Chromeleon Xpress These includ...

Page 42: ...2 13 immediately selects the Locked Remote mode In this mode you cannot change any operating parameters from the front panel keyboard To return the AS to Local mode clear the Connect check box on the...

Page 43: ...olume Align the sampling needle in the inject port Calibrate and test the leak sensor Monitor usage of replaceable parts see the description of Predictive Performance below Wellness panels for calibra...

Page 44: ...ve Performance commands and parameters are available in the Chromeleon Commands dialog box press the F8 key or select Command on the Control menu to open the Commands dialog box You can view and reset...

Page 45: ...and before performing the method s timed events The continue command can be sent via the front panel Hold Run button or a TTL input signal The wait s position in the sample prep steps determines wheth...

Page 46: ...hromeleon or Chromeleon Xpress If Chromeleon or Chromeleon Xpress is controlling the AS front panel settings for the wait operation are ignored Instead the Sample Overlap option determines whether sam...

Page 47: ...ing additional commands This allows the AS to act as the system master the AS controls the system by sending TTL signals to start the pump and detector methods To disable the wait operation press Menu...

Page 48: ...n is never shorter than the specified time However cycle time will be ignored thus delaying injection if any of the following events occurs WAIT FOR TEMP STABLE is enabled in the METHOD SETUP screen s...

Page 49: ...s During a Schedule Figure 2 19 shows the order of events during a schedule when the wait operation is enabled Figure 2 20 shows the order of events during a schedule when the wait operation is disabl...

Page 50: ...etc Method Setup Execute INIT Step Load Loop Method Time 0 0 Inject Method Times x x to x x Set TTLs Relays etc See Note 1 Sample Prep Overlap Method Finished Wait for Previous Method to Finish Method...

Page 51: ...nject Port and Wash Needle Do Sample Prep Pipetting Mixing etc Setup Method Execute INIT Step Load Loop Method Time 0 0 Inject Method Times x x to x x Set TTLs Relays etc See Note 1 Sample Prep Overla...

Page 52: ...es The INJ field is updated when the AS loads the loop for the next injection The TIME field and the METHOD timed events clock are reset to zero at injection The TIME field counts up from the injectio...

Page 53: ...pling needle arm moves to the waste port and expels any fluid from the needle The sampling needle arm moves to the inject port and delivers a volume of fluid for flushing the inject port The sampling...

Page 54: ...dle assembly see Figure 2 22 When the sample preparation option is installed additional priming is required to prime the lines from the reagent reservoir s to the sample syringe see Figure 2 24 See Se...

Page 55: ...w is between ports A F B C and D E Figure 2 24 shows the fluid connections to the sample syringe flush reservoir sample prep syringe and reagent reservoirs when the sample prep option is installed Fig...

Page 56: ...ple Syringe Valve Reagent Holding Loop Sampling Needle Line Reagent A Flush Reservoir Sample Syringe Sampling Needle A F B C D S E L N A B C D E F Back Fitting Front Fitting Front Fitting To From Need...

Page 57: ...screen is displayed the AS performs initialization procedures and a series of diagnostic tests If one of the tests fails testing stops and the DIAGNOSTIC TEST screen is displayed See Section C 9 2 if...

Page 58: ...ximum Level to Fill Vials 2 Screw a cap onto each vial and tighten To prevent septa from falling into the vials when they are pierced by the sampling needle make sure the cap septa are pushed fully in...

Page 59: ...6 Close the autosampler compartment door Make sure the door remains closed during operation Figure 3 2 Installing Tray Covers Sample Temperature Control Option Only If the door is opened during operat...

Page 60: ...nd the maximum sample volumes available when the well is filled NOTE Changing the needle height is not recommended when sampling from a micro well well plate because the well depth is shallow You can...

Page 61: ...y with the thumbscrews in the center of the tray and the arms across both well plates 9 Tighten the thumbscrews on the clamp snugly 10 Install the tray in the autosampler compartment Always install tw...

Page 62: ...ple into the wells Check the orientation of the cover to make sure the notches in the corners of the cover and well plate are aligned Press the well plate cover tightly into each well To avoid contami...

Page 63: ...panel When the AS is under computer control the front panel MAIN and DETAIL screens indicate this by displaying LOCKED RMT and DIRECT CONTROL in the operating and control mode fields See the example...

Page 64: ...click Start on the taskbar and select All Programs or Programs depending on the operating system Chromeleon Server Monitor Click Start to start the server 2 Start the Chromeleon or Chromeleon Xpress...

Page 65: ...peration and Maintenance Doc 065051 03 1 08 53 d Verify that the Connect check box is selected If it is not click the box to connect the AS to Chromeleon or Chromeleon Xpress Figure 3 6 AS Control Pan...

Page 66: ...ated automatically with the help of a software wizard or manually by editing an existing program In addition to commands for loading and injecting the sample a program for the AS can include commands...

Page 67: ...nject position All off 0 Schedule Missing Vial Action Vial Start End Injections per Vial Injection Volume Method Number Stop 1 1 1 25 0 L 1 mL for simultaneous injection 1 System Parameters Wait Syrin...

Page 68: ...ions For the 49 position vial tray vial position 1 is in the lower left corner of the tray and vial position 49 is in the upper right corner see Figure 3 7 Figure 3 7 49 Position Vial Tray For the 100...

Page 69: ...numbers are printed on well plates and well plate covers For example well plate position LA1 identifies the well located at position A1 in the well plate installed on the left side of the AS well pla...

Page 70: ...lume in the Chromeleon or Chromeleon Xpress sequence see Figure 3 10 and Figure 3 11 or in the front panel schedule see Section D 3 For volumes less than 100 L specify the value in 0 1 L Syringe Size...

Page 71: ...Doc 065051 03 1 08 59 increments For volumes of 100 L or greater specify the value in 1 L increments Figure 3 10 Sequence Wizard Specifying the Injection Volume Figure 3 11 Sequence Control Panel Spec...

Page 72: ...on to one system Partial LS Partial loop limited sample injection to one system no extra sample is aspirated Large Loop Large loop injection to one system Simultaneous Full loop injections to two syst...

Page 73: ...see Figure 3 12 and on the DETAIL STATUS screen see Figure 3 13 Figure 3 12 Injection Type Shown on Control Panel Figure 3 13 Injection Type Shown on Detail Status Screen Help prompt INJECTING VIAL TI...

Page 74: ...to two systems simultaneously a sample loop is installed on both injection valves Sequential Sample is delivered to two systems alternately a sample loop is installed on both injection valves Sequenti...

Page 75: ...jection volume and loop size see Table 3 4 The cut volume which is used only for partial loop and partial loop limited sample injections specifies a volume of sample to be cut or omitted from each sid...

Page 76: ...er the injection volume in the Chromeleon or Chromeleon Xpress sequence see Figure 3 16 or in the front panel schedule see Section D 3 Figure 3 15 Plumbing Configuration Screen Figure 3 16 Sequence Wi...

Page 77: ...forms a partial loop or partial loop limited sample injection 3 Enter the cut volume in the Chromeleon or Chromeleon Xpress program see Figure 3 17 or on the SYSTEM PARAMETERS screen see Section C 6 5...

Page 78: ...xamples for full loop large loop and partial loop injections The examples show the amount of sample used and injected for various loop sizes and injection volumes Injection Type Sample Volume Aspirate...

Page 79: ...but the amount actually injected is equal to the loop size Installed Loop Size L Injection Volume from Chromeleon Sequence L Sample Aspirated from Vial or Well L a a Excess aspirated sample is used to...

Page 80: ...sembly are installed If the sample loop is too large the sample volume drawn exceeds the syringe sampling needle assembly volume causing sample to enter the flush bottle To prevent sample from contami...

Page 81: ...gardless of the syringe size For syringes that are 1 000 L or smaller the maximum loop size and injection volume requires the syringe to aspirate from the sample vial multiple times This can result in...

Page 82: ...filled and an additional portion remains before the loop The sample in the loop is then injected onto the column see Figure 3 18 Figure 3 18 Full Loop Injection Sequence Sample Syringe 1 Draw air 3 D...

Page 83: ...Loop Injection Notes Entering an injection volume equal to or greater than the sample loop size sets up the AS for a full loop injection The actual volume of sample injected equals the volume of the...

Page 84: ...loop injection is the same as described for a normal full loop injection see Section 3 6 5 However when a large loop 200 0 to 7000 0 L is installed the AS draws a set amount 500 L of extra sample from...

Page 85: ...ple is positioned in the loop and injected see Figure 3 20 Figure 3 20 Partial Loop Injection Sequence Sample Syringe 1 Draw air 3 Draw air again to isolate the sample KEY Eluent Sample 6 Deliver samp...

Page 86: ...nt panel schedule see Section D 3 enter an injection volume that is less than the volume of the sample loop installed on the injection valve 3 In the Chromeleon or Chromeleon Xpress program see Figure...

Page 87: ...tion the AS aspirates 90 L from the sample vial the inject volume plus double the cut volume and delivers it to the injection valve Then enough flush fluid is drawn into the syringe to position the sa...

Page 88: ...rtial Loop Limited Sample Injection Sequence Sample Syringe 1 Draw air 2 Draw sample injection volume 3 Draw air again to isolate the sample 5 Prepare to load sample flush fluid air bubble is used KEY...

Page 89: ...of the sample loop installed on the injection valve 3 In the Chromeleon or Chromeleon Xpress program see Figure 3 21 or on the SYSTEM PARAMETERS screen enter a cut volume of zero Example Partial Loop...

Page 90: ...neous Injection Sequence 1 Draw air 3 Draw air again to isolate the sample KEY Eluent Sample Pump Column 5 Inject sample simultaneously onto both system s columns Empty sample syringe remaining sample...

Page 91: ...le syringe size of 5 000 or 10 000 L on the PLUMBING CONFIGURATION screen see Section C 6 3 On the SYSTEM PARAMETERS screen see Section C 6 5 select SIMULTANEOUS in the SAMPLE MODE field In the Chrome...

Page 92: ...er_InjectValve LoadPosition Switch the first inject valve to Load Sampler_InjectValve_2 LoadPosition Switch the second inject valve to Load Load Load sample onto the loop Wait CycleTimeState Wait for...

Page 93: ...jectState Wait for the injection to finish 0 000 Pump_InjectValve LoadPosition Switch the first inject valve to Load Pump_InjectValve_2 LoadPosition Switch the second inject valve to Load Load Load sa...

Page 94: ...ix elimination Figure 3 24 Concentrate Injection Sequence Example for Matrix Elimination 1 Draw air 3 Draw air again to isolate the sample 7 Inject sample onto column INJECT Pump Column Waste Waste 2...

Page 95: ...control select METHOD SYRINGE and FLUSH SYRINGE speeds on the SYSTEM PARAMETERS screen 5 For Chromeleon or Chromeleon Xpress control enter the syringe speed in the Program Wizard 1 is the slowest spee...

Page 96: ...the backpressure below 690 kPa 100 psi taking into account the pressure from the tubing and the concentrator column See Section 3 8 for details 6 In the Chromeleon or Chromeleon Xpress sequence see F...

Page 97: ...al CurrentVial AS sample prep commands Mix SourceVial CurrentVial NumberOfTimes 5 Volume 250 0 FlushSP Volume 250 0 DelaySP Time 0 0 Dispense Volume 1000 0 SourceReservoir Reservoir_A DestinationVial...

Page 98: ...e chromatography systems Each system is operated independently and can have a different sample and sample volume injected NOTE Precision for partial loop injections may be slightly affected by the lon...

Page 99: ...Prepare to load sample excess sample used Diverter valve selects inject valve 1 or 2 KEY Eluent LOAD Pump Column 6 Inject sample onto column Empty syringe remaining sample air bubble INJECT Pump Colum...

Page 100: ...inject port through the diverter valve to the injection valve see Section 5 9 3 On the SYSTEM PARAMETERS screen select SEQUENTIAL in the SAMPLE MODE field see Figure 3 27 4 On the PLUMBING CONFIGURAT...

Page 101: ...ding and injecting when the autosampler is in sequential mode Sampler AcquireExclusiveAccess The timebase acquires exclusive control of the AS to prevent another timebase from attempting to control th...

Page 102: ...limination 1 Draw air 3 Draw air again to isolate the sample 2 Draw sample inject volume Sample Syringe LOAD Pump Column Waste Concentrator L P C L S W Inject Valve 1 Inject Valve 2 Pump Column L P C...

Page 103: ...on valves on two systems 2 Install a concentrator column on each injection valve 3 Calibrate the inject port volume of each system This is the volume from the inject port through the diverter valve to...

Page 104: ...n 3 8 to select the appropriate syringe speed syringe size and tubing IDs to keep the total backpressure below 690 kPa 100 psi Selecting Separate Aspirate and Dispense Speeds If you are using Chromele...

Page 105: ...red speeds for aspirating and dispensing liquids during priming and flushing 1 is the slowest speed and 5 is the fastest 2 To select aspirate and dispense speeds for loading the loop click the More Sy...

Page 106: ...rogram NOTE If you do not include aspirate and dispense syringe speed commands in a program the autosampler uses the speeds selected in the previous program or on the Control panel whichever are the m...

Page 107: ...ter the Wait FlushState command and press the Enter key twice to create two empty lines 3 Press the F8 key to open the Commands dialog box 4 Under Sampler enter a value for the LoadDispenseSpeed see F...

Page 108: ...ispense syringe speeds for the Concentrate Reagent Prime and Reagent Flush functions See Section 3 6 10 for details about concentrate mode NOTE The SyringeSpeed command shown in the program in Figure...

Page 109: ...mm ID tubing x 1 6 kPa mL min cm x 2 40 mL min 5 mL syringe at speed 2 115 kPa Tubing ID Backpressure in kPa psi per mL min per cm of tubing Volume in L per cm of tubing 0 127 mm 0 005 in 25 kPa 3 6 p...

Page 110: ...500 and 1 000 L 1 0 03 2 0 09 3 0 24 4 0 60 5 1 20 2 5 5 and 10 mLa 1 1 80 2 2 40 3 4 80 4 7 20 5 15 00 Table 3 12 Flow Rates Provided by the Prep Syringe a In general if you are using a 5 or 10 mL s...

Page 111: ...Pa Therefore the selected tubing IDs tubing lengths and syringe speed can be used Tubing Tubing Length cm Backpressure from tubing kPa mL min cm Flow Rate mL min Backpressure Produced kPa Needle Seal...

Page 112: ...e one of the following messages appears The message depends on whether a Chromeleon or Chromeleon Xpress sequence or a front panel schedule is running Figure 3 36 Open Door Safety Screen A Chromeleon...

Page 113: ...mputer control the door can be opened safely only when the sampling arm is in the home position and the autosampler is idle This can occur at various times in a program for example after injection is...

Page 114: ...nce procedures must be performed by Dionex personnel 3 10 1 Daily Check for air bubbles in the syringe s and tubing and remove them if they appear see Section B 3 14 Check the volume of liquid in the...

Page 115: ...he Chromeleon Wellness panel displays the usage counters for the various parts Replace or service parts when recommended and reset the usage counter afterwards see Section 5 2 1 3 10 3 Annually Perfor...

Page 116: ...shutdown procedure is required If the AS is under Chromeleon or Chromeleon Xpress control you can load a shutdown program at the end of the schedule to automatically shut down the system The shutdown...

Page 117: ...is identified by a number in brackets in the lower right corner of the AS screen To clear the message press any key The Moduleware the instrument control firmware installed in each AS also reports er...

Page 118: ...oes not acknowledge The DSP digital signal processor did not respond to a command from the CPU Possible Causes Communication problem between the DSP and the CPU Actions Turn the power off and then bac...

Page 119: ...he AS rear panel see Section B 3 3 If the prep syringe is installed check its cable connection also Rerun the test If the SYRINGE COMM test still fails or if other tests fail contact Dionex 362 Syring...

Page 120: ...75 An attempt was made to use the sample prep syringe This option is not installed Two of the method sample prep operations DISPENSE and DILUTE can be used only when the sample preparation option is i...

Page 121: ...e lines if necessary see Section 5 1 Check the syringe motion If the syringe action is faulty the syringe may need to be replaced see Section 5 12 If the syringe does not move in response to a command...

Page 122: ...Possible Causes Problem with the Moduleware Actions Turn off the AS power and then turn on the power again If the message reappears contact Dionex 382 An attempt was made to move a syringe valve to a...

Page 123: ...es assuming a tray is installed but is not recognized by the sensor Tray magnets are missing or incorrectly installed Sensors are not working Actions Replace the tray Replace the tray sensor card Cont...

Page 124: ...r and then turn on the power again and recheck the INSTALLED OPTIONS screen If the problem persists contact Dionex 389 Timed out waiting for motion complete command from DSP The DSP could not complete...

Page 125: ...Setting system parameters to defaults and initializing schedule and method database Possible Causes Temporary short on CPU board Dead battery on CPU board Malfunctioning power supply Actions If a temp...

Page 126: ...not during diagnostics the syringe can perform up to four multiple strokes to aspirate or dispense a volume up to four times larger than the syringe size Possible Causes During a schedule or flush pri...

Page 127: ...ion in the autosampler compartment 3 Check for freedom of movement in all three axes of the sampling arm 4 Turn on the power again If the problem persists contact Dionex 421 Timed events step took so...

Page 128: ...Reinitializing to default values Possible Causes Dead battery on CPU board Malfunctioning power supply Actions Contact Dionex 431 Format of calibration database has changed Reinitializing to default v...

Page 129: ...lfunction Actions Close the autosampler compartment door and then press the Home button If closing the door does not eliminate the problem try turning the power off and then on again If the problem pe...

Page 130: ...he inside lower front edge of the door is present Also make sure the magnet on the lower edge of the autosampler compartment is present If a magnet is missing contact Dionex 443 Leak detected in tray...

Page 131: ...thoroughly and recalibrate see Section 5 11 447 Leak sensor needs recalibration Go to Leak Sensor Calibration screen to perform recalibration operation Actions See Section 5 11 for calibration instru...

Page 132: ...ossible Causes An error occurred during Z axis movement of the sampling arm The DSP was able to recover from the error and continue the motion Actions Occasional occurrences of this error can be ignor...

Page 133: ...ible Causes Valve in wrong position Actions Turn off the AS power and then turn on the power again If the message reappears contact Dionex 455 Recoverable motion error occurred in auxiliary valve Poss...

Page 134: ...e error Both internal and external inject valves are connected Please power down and disconnect one inject valve before continuing Possible Causes An injection valve is installed in both the autosampl...

Page 135: ...an exhaust in the back for any obstructions restricting airflow Make sure there is at least 6 cm 2 5 in of free space behind the AS for ventilation If the problem persists contact Dionex 482 An inject...

Page 136: ...the leak is from one of the valve ports remove the valve and syringe see Section 5 12 Remove the existing white Teflon washer from inside the valve port and replace it with a new washer P N 061315 The...

Page 137: ...ion 5 4 Check the needle seal if the seal appears worn or damaged replace the needle seal assembly see Section 5 7 If fluid appears on the top of the inject port during injection the needle may not be...

Page 138: ...covers of the micro well plates if the tray temperature set point is at 4 C and the ambient temperature and relative humidity in the laboratory are above 25 C and 60 humidity To prevent condensation...

Page 139: ...disposal or recycling practices in your area 5 1 Replacement Tubing and Fittings The following tables list the tubing fittings and tubing assemblies used to plumb the AS autosampler compartment NOTE T...

Page 140: ...sampling needle arm to the sampling valve use with sample syringe volumes of 1 mL or less 061267 Sampling needle assembly with an 8 5 mL volume Connecting the sampling needle arm to the sampling valv...

Page 141: ...e Section 2 6 for more information about Predictive Performance Chromeleon monitors usage of the following AS parts injection valve diverter valve XYZ sampling needle arm sampling needle assembly need...

Page 142: ...bset in Chromeleon or Chromeleon Xpress and select the Autosampler tab 2 Click the Wellness panel button to open the AS Wellness Panel see Figure 5 1 3 Click the Reset button for the part that you rep...

Page 143: ...the cursor in the RESET field of the part that was replaced or serviced and press Enter The number is reset to zero Figure 5 2 Usage Log Screen Figure 5 3 Usage Log Screen Additional Parameters Help P...

Page 144: ...isplays the number of cycles performed since the syringe was last replaced The table below lists part numbers for the available syringe sizes Syringe Size Part Number 100 La a The 100 L syringe is for...

Page 145: ...at the top and bottom and unscrew it from the valve 3 Remove the white Teflon washer from the syringe valve port Note To remove the washer push a piece of 0 8 mm 0 031 in OD PEEK tubing into the cent...

Page 146: ...fill the syringe completely 5 Remove the syringe from the liquid While holding it vertically push a small amount of liquid out the top to make sure no air remains Method B 1 Fill a container with isop...

Page 147: ...bbles see Section 5 3 2 Reconnect the syringe and flush again 5 3 4 Initializing the Syringe 1 On the MENU of SCREENS press 5 and 3 to go to the PLUMBING CONFIGURATION screen Figure 5 5 Flush Prime Sc...

Page 148: ...eplacing the syringe reset the syringe usage counter from either the AS Wellness panel in Chromeleon or Chromeleon Xpress or on the front panel USAGE LOG SCREEN see Section 5 2 5 4 Replacing the Sampl...

Page 149: ...includes the same parts as the 1 2 mL assembly plus a coupler and an additional length of tubing to increase the volume see Figure 5 9 NOTE The PEEK sampling needle assemblies use a different bolt an...

Page 150: ...ect FLU and press Enter The needle arm moves to the flush port 3 Turn off the power and open the autosampler door Figure 5 9 8 5 mL PEEK Sampling Needle Assembly P N 061267 PEEK Needle Yellow Sleeves...

Page 151: ...s have a clip that is open at the top This type of clip does not need to be removed To remove the tubing from the clip pull the clip out slightly and push the tubing up and out of the clip 5 Measure t...

Page 152: ...e sleeve on the new line the same distance from the needle Figure 5 11 Sampling Needle Line Bracket and Clamp Right Side of Compartment 8 If you are replacing an 8 5 mL assembly P N 061267 go on to St...

Page 153: ...ou are replacing an 8 5 mL sampling needle assembly P N 061267 disconnect the sampling needle line from the coupler that connects it to the larger ID tubing see Figure 5 11 NOTE The coupler may be hid...

Page 154: ...ng the needle making sure it is lined up with the opening in the cradle The needle stops when the fitting on the needle reaches the bottom of the needle block 3 Tighten the fitting fingertight 4 Posit...

Page 155: ...clamp leaving about 45 cm 18 in free 9 If you are replacing an 8 5 mL sampling needle assembly P N 061267 follow the steps below If you are replacing a 1 2 mL sampling needle assembly go on to Step 1...

Page 156: ...d of the tubing c Insert the tubing with the bolt and ferrule into port C on the sample syringe Push the tubing snugly into the bottom of the port d While holding the tubing with one hand to avoid twi...

Page 157: ...sampler compartment and remove the injection valve waste line from the opening in the tray see Figure 5 15 2 Pull out the tray toward the front of the autosampler about 6 mm 1 4 in 3 Tilt the bottom o...

Page 158: ...ld be at least a paper width gap between it and the tray 2 Tilt the top of the tray in and the bottom out and partially slide the tray back into the autosampler Make sure the leak sensor clears the fr...

Page 159: ...und opening at the right front corner of the drip tray 2 Insert the waste line from the second injection valve into the larger drain line opening see Figure 5 18 Figure 5 17 Routing the Injection Valv...

Page 160: ...t the needle seal line fitting from port S 5 on the injection valve Using a 5 16 in wrench turn the fitting counterclockwise to loosen it and then complete the removal with your fingers If the AS is e...

Page 161: ...ntil the slots in the needle seal align with the projections on the top of the inject port see Figure 5 21 Figure 5 21 Removing the Needle Seal Assembly 4 Push up from underneath and slide the entire...

Page 162: ...el in Chromeleon or Chromeleon Xpress or on the front panel USAGE LOG SCREEN see Section 5 2 11 Align the sampling needle in the inject port see Section 5 8 and then calibrate the inject port volume s...

Page 163: ...needle to make sure it is not bent If the needle is bent replace the sampling needle assembly see Section 5 4 Two methods are available for aligning the sampling needle Automatic alignment using Chrom...

Page 164: ...152 Doc 065051 03 1 08 To align the sampling needle in Chromeleon 1 Open the Chromeleon Browser and open a panel tabset 2 Select the Autosampler tab to open the AS Control panel see Figure 5 24 Figur...

Page 165: ...the following notes Step 1 For a better view of the needle and inject port during the alignment procedure you can open the autosampler door after clicking Inject port align Figure 5 25 Chromeleon Nee...

Page 166: ...se the needle s z axis height and click Move to move the needle down Note Each digit in the z axis height value moves the needle 0 127 mm 0 005 in Step 4 After clicking Check the AS dispenses 500 L of...

Page 167: ...Chromeleon or Chromeleon Xpress If Chromeleon or Chromeleon Xpress Release 6 8 SP2 or later is unavailable refer to Section D 9 for instructions on how to use the front panel screens to calibrate the...

Page 168: ...e Calibration panel appears The panel and calibration procedure differs depending on whether the AS is configured for normal or sequential mode If the AS is in normal mode refer to the instructions on...

Page 169: ...e outside the autosampler or chromatography compartment Make sure the sample syringe is free of bubbles see Section B 3 14 Step 1 Clicking Setup first empties the needle seal line The AS then dispense...

Page 170: ...han was cleared You can then begin clicking 1 uL again Step 3 Press Reset and then Test After clicking Test verify that a drop of liquid appears at the end of the needle seal line If no drop appears o...

Page 171: ...er valve lines from the injection valves route the lines outside the autosampler or chromatography compartment Make sure the sample syringe is free of bubbles see Section B 3 14 Step 1 Select Diverter...

Page 172: ...before clicking 1 uL again Continue clicking 1 uL until a tiny drop of liquid appears at the end of the diverter line If you go too far and more than a single small drop of liquid appears click 5 uL...

Page 173: ...w leak sensor P N 053669 4 Attach the sensor to the drip tray push the cable inside the autosampler compartment and reinstall the drip tray see Section 5 6 NOTE Make sure the bottom wire on the leak s...

Page 174: ...l 1 Press Menu 8 and 0 to go to the LEAK SENSOR CALIBRATION AND STATUS screen Figure 5 29 Leak Sensor Calibration and Status Screen 2 Press Select or Select to select CAL and then press Enter The curr...

Page 175: ...rols on the Wellness panel are enabled see Figure 5 30 5 Under Calibration leak detector click tray The AS calibrates the sensor and then uploads the new value to Chromeleon Chromeleon stores the new...

Page 176: ...3 Press Select to select the INITIALIZE option 4 Press Enter The syringe plunger moves to the initial position and the syringe motor shuts off 5 Unscrew and remove the syringe drive connecting screw a...

Page 177: ...installed If the slot in the drive shaft is not in the correct position the syringe drive may need replacing Contact Dionex for assistance Do not attempt to manually turn the drive shaft 4 Check the...

Page 178: ...D at the 12 o clock and 6 o clock positions respectively push the valve onto the syringe drive shaft 9 Replace the two valve thumbscrews and partially tighten 10 Carefully align the syringe piston wit...

Page 179: ...y need replacing Contact Dionex for assistance Do not attempt to manually turn the drive shaft 4 Check the back of the new prep syringe valve P N 054243 The valve drive shaft should be perpendicular t...

Page 180: ...B at the 12 o clock position and the syringe at the 6 o clock position push the valve onto the syringe drive shaft 8 Replace the two valve thumbscrews and partially tighten 9 Carefully align the syri...

Page 181: ...P N 062379 and sample prep syringe P N 062365 1 Turn off the AS power 2 Disconnect the liquid lines from the existing syringe valve 3 Unplug the syringe cable from the rear panel connector 4 Lift up...

Page 182: ...e number of cycles reaches this usage limit If the AS is operating under front panel control the USAGE LOG SCREEN see Section C 9 6 displays the number of cycles performed since the valve was last reb...

Page 183: ...r screws the last step in the valve rebuild kit instructions Before tightening the stator screws make sure the stator and the stator ring are squarely aligned and the space between them is uniform all...

Page 184: ...are shipped with protective covering on both sides One side is clear and the other is patterned Peel off the clear covering from the pad 6 Note the two slightly recessed rectangles on the well plate...

Page 185: ...holder straight out of its compartment Figure 5 40 Autosampler Compartment Main Power Fuse Holder 3 The holder contains two fuses Replace both with new 3 15 amp fast blow IEC 127 fuses P N 954745 Dio...

Page 186: ...r into its compartment The fuse holder is keyed to fit only in its proper orientation Apply sufficient pressure evenly against the holder to engage the two locks The holder is flush against the panel...

Page 187: ...amps The AS power supply is main voltage auto sensing and requires no adjustment Fuses Two 3 15 amp fast blow IEC 127 fuses P N 954745 Ambient Operating Temperature 10 to 40 C 50 to 104 F Operating H...

Page 188: ...olume Range If the sample syringe is 1 000 L or less 1 0 to 99 9 L in 0 1 L increments 100 to 1 000 L in 1 L increments normal or sequential mode 100 to 1 000 L in 1 L increments concentrate or sequen...

Page 189: ...d other sizes are available Concentrator Column Dionex ultra low pressure maximum injection pressure is 690 kPa 100 psi Maximum Pressure Delivery 690 kPa 100 psi Temperature Control Range 4 to 60 C in...

Page 190: ...AS Autosampler Operator s Manual 178 Doc 065051 03 1 08...

Page 191: ...t P N 062380 or P N 062381 B 1 Facility Requirements Make sure the AS meets the power and environmental specifications listed in Appendix A Install the AS on a sturdy workbench at a height that ensure...

Page 192: ...l armoire Son soul vement par la porte du panneau avant endommagera les charni res de la porte Der AS wiegt ber 18 kg Daher sollte das Ger t nur von zwei oder mehr Personen angehoben werden Greifen S...

Page 193: ...radle Note The AS is inserted into the bottom foam of the main shipping container The tubing is coiled in front of the AS 3 Using the handholds in the cradle place the AS on the bench 4 Remove the sle...

Page 194: ...er Note The rear of the AS is aligned with the rear edge of the foam Figure B 8 5 cm 2 in wide masking tape securing tubing within the recess at the rear of the AS 6 Remove the masking tape 7 Remove t...

Page 195: ...h pigtails in bags that are closed with sturdy ties Syringes packed with foam insert placed between them Handles are tied together with sturdy ties Figure B 12 Dual syringes with bag removed Note For...

Page 196: ...Fully assembled dual syringes with bottle Figure B 14 Shipping bracket hooked into frame and attached to sample arm and chassis with screws 13 Remove the sturdy tie the two cap screws and the bracket...

Page 197: ...ve the clip and sleeve You will need them in the future if the autosampler is shipped Figure B 17 Polyethylene sleeve and clip Figure B 18 Shaft with polyethylene sleeve removed Note Remember to save...

Page 198: ...omatography Management System with a Dionex Ion Chromatograph Document No 031883 for instructions B 3 2 Installation Checklist Verify that all shipping restraints are removed Connect the syringe s and...

Page 199: ...the sample syringe 2 Remove the 1 4 28 plug from port C on the sample syringe valve Make sure the white Teflon washer remains installed inside the port 3 Connect the sampling needle assembly line to p...

Page 200: ...f the drain line that extends from the lower right front corner of the autosampler compartment see Figure B 20 or sample temperature control option into a waste container Figure B 20 Autosampler Compa...

Page 201: ...the cable from the prep syringe to the PREP SYRINGE connector on the rear panel Neutralisez les d chets acides ou caustiques avant de les jeter Jetez les d chets aux r glements locaux Neutralisieren S...

Page 202: ...the line that extends from port A on the prep syringe valve to the reservoir cap see Figure B 22 3 If you are installing additional reagent reservoirs connect the lines from ports B C and or D to the...

Page 203: ...e that extends from the inject port in the autosampler is coiled inside the AS door on the right side Route this line through the side slot on the AS see Figure B 23 and then through the side slot on...

Page 204: ...e side slot on the DC and then through the side slot on the AS 6 If you are connecting the AS to an ICS 1000 1500 2000 this green waste line labeled AS TRAY is connected with a union to a Teflon waste...

Page 205: ...e small round opening on the right side of the AS tray see Figure B 23 B 3 5 Connecting Two Injection Valves for Simultaneous Injections Connecting to Two Valves Installed in Chromatography System Mod...

Page 206: ...ip tray Insert one line into the small round opening in the tray see Figure B 23 Insert the other line about 12 mm 1 2 in into the large drain opening in the tray Figure B 27 Injection Valve Connectio...

Page 207: ...port P 2 on the bottom injection valve valve 1 and connect the column outlet line from system 1 to port C 3 on valve 1 see Figure B 29 3 Connect the pump outlet line from system 2 to port P 2 on the t...

Page 208: ...nections for Simultaneous Injection Valves Installed in the AS Make sure the injection valve waste lines are not elevated above the injection valves at any point between the valves and the AS drip tra...

Page 209: ...available In addition to the kit you will need a 5 5 mm nut driver to complete the installation Installing the Diverter Valve 1 Connect the cable from the diverter valve see Figure B 31 to the matchi...

Page 210: ...ounted on the right side of the autosampler compartment see Figure B 32 and push the assembly onto the studs see Figure B 33 4 Make sure the cable is not pinched and then slide the assembly back to se...

Page 211: ...verter valve to port S on the injection valve of system 1 see Figure B 35 Figure B 34 Diverter Valve Plumbing Connections Figure B 35 Diverter Valve Plumbing Connections to Injection Valves Needle Sea...

Page 212: ...configured with two injection valves two AS Autosamplers can be connected to the DC The first AS is installed on the left side of the DC and the second AS is installed on the right side A Second AS Ki...

Page 213: ...hed when the door is closed Figure B 37 Second AS Installation Needle Seal Line Routed Out the Left Side of the AS 6 Align the sampling needle in the inject port see Section 5 8 7 Calibrate the inject...

Page 214: ...e side slot on the AS 11 To ensure the green waste line stays in place in the opening in the AS drip tray sharply bend the line about 12 mm 1 2 in from the end being careful not to restrict the inner...

Page 215: ...th a Dionex Ion Chromatograph Document No 031883 for instructions The manual is available in two formats as a printed manual shipped with Chromeleon or Chromeleon Xpress and as an Adobe PDF file on th...

Page 216: ...2381 2 Plug the B connector of the USB cable into the USB receptacle on the rear panel of the AS 3 Plug the A connector of the cable into the USB port on one of the following locations a module with a...

Page 217: ...B receptacle on the AS Plug the A connector into an A port on the USB hub USB cable s connector Plug a A into a USB port on the PC Plug the B connector into the B receptacle on the USB hub USB cable s...

Page 218: ...t AC input levels outside of the specified operating voltage range may damage the AS DANGER D LECTROCUTION Pour viter toute lectrocution il faut utiliser une prise de courant avec prise de terre Ne l...

Page 219: ...iefly and then the POWER UP screen appears While the POWER UP screen is displayed the AS performs initialization procedures and a series of diagnostic tests If one of the tests fails testing stops and...

Page 220: ...decrease the current values 3 Move the cursor to the DATE field 4 Press numeric buttons to enter the day and year directly or press Select or Select to increase or decrease the current values Press S...

Page 221: ...CRIPTION NORMAL default Injections to a single system CONCENTRATE Injections to a single system when the sample loop is replaced by a concentrator column SIMULTANEOUS Injections to two systems simulta...

Page 222: ...mbing Configuration Options 1 On the MODULE SETUP MENU press 3 to go to the PLUMBING CONFIGURATION screen see Figure B 44 2 Move the cursor to the SAMPLE SYRINGE VOLUME field and select the volume of...

Page 223: ...hromeleon Xpress The software enables the wait function automatically and ignores the setting on the SYSTEM PARAMETERS screen 1 Go to the MODULE SETUP MENU and press 5 to go the SYSTEM PARAMETERS scre...

Page 224: ...or detailed instructions on how to configure the AS in the software In summary Install the software enter the license and connect the USB cable before turning on the AS power When you start the AS aft...

Page 225: ...Xpress setup instructions After configuring the AS in one or two timebases you can start Chromeleon or Chromeleon Xpress and connect to a Control panel see Figure B 46 For Chromeleon Xpress connectio...

Page 226: ...Control panel see Figure B 47 2 Enter the Prime Volume Refer to Table B 2 or Table B 3 for the suggested volumes 3 If the sample preparation option is installed select Prep in the Prime Syringe list...

Page 227: ...ions Suggested Priming Volumes Sample Syringe Size Suggested Priming Volume 1 000 L 2 000 to 2 500 L 5 000 L 9 999 La a The maximum value that can be entered 10 000 L 19 998 Lb b Run two priming cycle...

Page 228: ...yringe Valve Flush Reservoir Sample Syringe Sampling Needle A B C D E F Sampling Needle Assembly Prep Syringe Reagent B Optional Reagent C Optional Reagent D Optional Prep Syringe Valve Sample Syringe...

Page 229: ...ringe If the sample preparation option is not installed the PRIME RESERVOIR and PRIME SYRINGE fields default to FLUSH and SAMPLE respectively and cannot be edited 5 Move the cursor to the PRIME ACTION...

Page 230: ...plunger to draw a full syringe volume into the syringe Figure B 51 Removing the Syringe 6 Keeping the syringe in the liquid slowly push the syringe plunger until all bubbles exit the syringe Then fill...

Page 231: ...857 is installed between ports 1 and 4 Other sample loop sizes are available Contact Dionex for information Figure B 52 shows the valve connections This information is for reference should you need to...

Page 232: ...tector Pump_ECD for a combined pump and detector and Sampler for an autosampler In addition other components relays TTLs injection valves etc are assigned device names also If you assign multiple devi...

Page 233: ...B Installation Doc 065051 03 1 08 221 In the example below the device name for the second ICS 2000 in a timebase is renamed Pump_ECD_2 This device will be identified as System 2 on the Control panel...

Page 234: ...me signals and other devices associated with System 2 For example if System 2 is an ICS 2000 as in the previous example select the Signals tab click Change and add _2 to the signal name Repeat for the...

Page 235: ...B Installation Doc 065051 03 1 08 223 Also select the State Devices tab and add _2 to the name of each enabled device Press F2 to edit a device name See the following example...

Page 236: ...ile for Systems Sharing an AS For simultaneous injection analyses create a single method file with all components for each detector channel included in one component list Use the Duplicate Column comm...

Page 237: ...sociated with the anion detector channel and displays only the anions If two anion or two cation detector channels were being used the measured retention time for each anion or cation would appear in...

Page 238: ...AS Autosampler Operator s Manual 226 Doc 065051 03 1 08...

Page 239: ...ODE VERSIONS USAGE LOG 2 2 2 1 1 1 1 3 3 4 5 2 2 3 3 4 5 4 6 5 7 7 6 8 8 9 9 1 TIME FUNCTION OUT POWER UP DIAGNOSTIC TESTS XYZ TEST TEMP STATISTICS LIQUID CONTROL LOGS MENU LEAK CAL STATUS MESSAGE LOG...

Page 240: ...each screen press Help NOTE If no keypad buttons are pressed within a 2 hour period the backlight automatically turns off To turn the backlight on again press any button on the keypad Press the butto...

Page 241: ...DING The schedule and method are on hold CHECKING FOR SAMPLE VIAL PRESENCE At the beginning of a method the AS checks that the specified sample vial is present PIPETTING The AS is pipetting from one v...

Page 242: ...ECUTING SETUP AND INIT STEP The AS is executing the method setup conditions and the INIT step of the method s timed events WAITING FOR CYCLE TIME TO EXPIRE The AS is waiting for the cycle time to expi...

Page 243: ...ay temperature A symbol to the right of this field indicates the temperature status see below If the sample temperature control option is not installed the field displays and it is not illuminated The...

Page 244: ...d the DETAIL STATUS screens Figure C 4 Detail Status Screen Control Mode SCH schedule or DIRECT CONTROL Press Select or Select to select SCH schedule or DIRECT CONTROL mode When the AS is in LOCKED RM...

Page 245: ...g the temperature status are displayed to the right of this The temperature is stabilized The temperature is increasing The temperature is decreasing The temperature control option is off Pressing Sel...

Page 246: ...le control set the TTL and relay fields from the TIMED EVENTS screen for the method being run Appendix E describes TTL and relay control 1 on 0 off INJ VLV Displays and lets you set the position of th...

Page 247: ...em NORMAL LS Limited sample injection to one system NORMAL LARGE Large loop injection to one system CONCENTRATE Concentrate injection to one system SIMULTANEOUS Full loop injections to two systems sim...

Page 248: ...reen s number and press Enter To return to this screen press Menu NOTE A method can have a maximum of 100 steps The total includes the sample prep steps the method setup which counts as one step and t...

Page 249: ...lute or a relative position To specify an absolute vial position press numeric buttons from 1 to 100 depending on the number of vial positions available in the currently installed tray To specify a re...

Page 250: ...ple syringe is always used for pipetting L Pipet volume from 1 0 to 99 9 L in 0 1 L increments or 100 to 1 000 L in 1 L increments default 20 L Source vial position the vial from which to pick up the...

Page 251: ...rvoir the reservoir from which reagent is dispensed RES A RES B RES C or RES D default RES A to Destination vial position the vial to receive the dispensed volume default SMP DILUTE sample prep option...

Page 252: ...ource enter either the vial from which to pick up the volume of reagent or select the flush reservoir FLH If the sample prep option is installed a reagent reservoir can be selected RES A RES B RES C o...

Page 253: ...ce is a vial the range depends on the volume of the sample syringe NOTE The REAGENT FLUSH and REAGENT PRIME operations are performed after the autosampler loads the sample onto the concentrator column...

Page 254: ...tions If WAIT FOR TEMP STABLE is enabled in the METHOD SETUP screen see Section C 6 and the tray or column temperature has not stabilized by the end of the cycle time If the wait step of the method s...

Page 255: ...ture control option is installed 4 to 60 C default off default set point 20 WAIT FOR TEMP STABLE Specifies whether the AS should wait until the temperature of the sample tray has stabilized before con...

Page 256: ...ections Only one LOAD and one INJECT command can be specified in a method Sample loading must occur before the injection By default LOAD occurs at the INIT step and INJECT occurs at TIME 0 00 LOAD INJ...

Page 257: ...SING VIAL ACTION The action the AS takes if a vial is missing from the tray Select SKIP to ignore the missing vial and move to the next vial in the schedule Select STOP to stop the schedule To resume...

Page 258: ...zero injections is specified only the sample prep portion of the method will be performed 0 to 99 default 1 INJ VOL L non simultaneous injection mode The volume of sample delivered for each injection...

Page 259: ...he autosampler compartment This allows all injection valve commands issued from Chromeleon or Chromeleon Xpress to control the valve in an IC system ICS 1000 ICS 1500 ICS 2000 or ICS 3000 The INJECTIO...

Page 260: ...he brightness of the display panel backlight The off option darkens the display completely When off press any button to turn the light on again LOW MEDIUM HIGH OFF default HIGH KEY ACTUATION SOUND Tur...

Page 261: ...mL This field is not available in concentrate mode 2 0 to 99 9 L 100 to x L default 25 LOOP SIZE V2 The volume of the loop installed on the second injection valve This field is available in simultane...

Page 262: ...default 5 000 SAMPLE SYRINGE CONFIG The action to be performed by the sample syringe These actions are used to configure the syringe at initial setup or after a new syringe is installed INITIALIZE mov...

Page 263: ...n seconds For example if the time is 8 35 a m and 50 seconds enter 08 35 50 If the time is 8 35 p m and 50 seconds enter 20 35 50 Press numeric buttons to enter the time directly or press Select or Se...

Page 264: ...ld Description Values WAIT Enables or disables the wait step in the sample prep phase of the method When wait is enabled the AS waits for a run command from either the front panel Hold Run button or a...

Page 265: ...efault value of 3 more viscous liquids require slower speeds For concentrate mode select a speed of 2 Be sure to select a speed that does not produce backpressure of more than 690 kPa 100 psi See Sect...

Page 266: ...690 kPa 100 psi See Section 3 8 for details In general if you are using a 5 or 10 mL syringe select a speed of 1 or 2 1 slowest to 5 fastest default 4 EMPTY SYRINGE SPEED This parameter is displayed...

Page 267: ...s an easy to use method and is recommended when Chromeleon or Chromeleon Xpress is available See Section 5 8 for detailed instructions Figure C 21 Inject Port Alignment Screen Screen Field Description...

Page 268: ...computer control Locked Remote Figure C 22 Door Interlock Bypass Screen Be aware of unexpected sampling arm movement when operating with the door open Soyez conscient de la possibilit de mouvements i...

Page 269: ...ection 3 7 If the AS is always controlled from the front panel select the syringe speed on the SYSTEM PARAMETERS screen see Section C 6 5 Screen Field Description DOORINTERLOCK BYPASS Press Select or...

Page 270: ...UME The volume of fluid used for priming 100 to 5 000 L default 400 PRIME RESERVOIR The reservoir used for the prime cycle If the sample preparation option is installed press Select or Select to scrol...

Page 271: ...the controlling device NORMAL EDGE the default mode is compatible with the TTL output signals provided by Dionex modules NORMAL EDGE INVERTED EDGE NORMAL PULSE INVERTED PULSE UNUSED TTL1 Not active ST...

Page 272: ...nnected and the serial number of the AS This is the same power up screen that displays when you turn on the AS Figure C 25 Diagnostic Menu Screen Figure C 26 Power Up Screen Help Prompt DIAGNOSTIC MEN...

Page 273: ...Tests Screen Test Description CPU Checks the CPU internal configuration and the Moduleware checksum LEAKS Checks whether the leak sensors detect a leak DSP COMM Checks communication between the CPU an...

Page 274: ...3 or 4 CYCLE Continuously cycles the arm through the four positions PATTERN Continuously cycles through a four corners test pattern with the needle moving about halfway into the vial VIALS Continuousl...

Page 275: ...numbers indicate the number of motor steps away from the home position X SENSOR Y SENSOR Indicates the current state of the X and Y sensors The X and Y sensors detect evenly spaced slots that indicat...

Page 276: ...select a value to monitor move the cursor to the desired field and press Enter TRAY SET TEMP The temperature to maintain in the tray ACTUAL Displays the measured temperature The reading is updated eve...

Page 277: ...ontrol Screen Simultaneous or Sequential Mode Help Prompt LIQUID CONTROL from VIAL ASPIRATE SYRINGE ACTION DISPENSE 1 100 100 SAMPLE ASPIRATE uL uL NEEDLE LOAD 46 NEEDLE HEIGHT SYRINGE SPEED 4 INJ VAL...

Page 278: ...efault 100 L SYRINGE The syringe to use for an action SAMPLE PREP ACTION The action to perform Press Select or Select to scroll through the choices press Enter to start the action ASPIRATE DISPENSE EM...

Page 279: ...counters after replacing a component The status of each parameter is updated in real time Figure C 32 Logs Menu Screen Figure C 33 Usage Log Screen Help Prompt LOGS MENU USAGE LOG MESSAGE LOG 1 2 Hel...

Page 280: ...nse cycles the prep syringe has performed since the counter was last reset to 0 Reset the counter after replacing the syringe XYZ NEEDLE ASSEM The number of XYZ sampling needle arm movements completed...

Page 281: ...e error code and a brief description of the error To see a full screen description of an error move the cursor to the error s entry number and press Help Press Select or Select to go to the beginning...

Page 282: ...f the sensor reading is within the wet range or ERR if the sensor is out of range If the measured value drops 0 02 volts below the calibration value the current condition is wet If the measured value...

Page 283: ...working correctly the button s display changes to reverse video 2 Continue pressing all buttons in turn Only the most recently pressed button shows in reverse video 3 To end the test and return to th...

Page 284: ...unction Out Screen Screen Field Description Values LOAD INJECT VALVE TTL1 TTL2 If the AS is not connected to Chromeleon or Chromeleon Xpress a TTL connection is required between the AS TTL output and...

Page 285: ...again to go to the SYSTEM PARAMETERS screen To return to the MAIN STATUS screen 1 Press Menu twice 2 Press Enter or 1 D 2 Entering or Changing Values NOTE You can enter or change screen field values...

Page 286: ...ection D 6 for details about creating schedules Notes The AS can run a schedule while you are entering or editing any method or schedule even the one that is currently running Changes made to the curr...

Page 287: ...s 1 through 9 5 Press Enter 6 To start the schedule press the Hold Run button NOTE You can also select the schedule from the SCHEDULE screen see Figure D 2 Enter the number in the RUN field and press...

Page 288: ...running the schedule press Hold Run NOTE If wait is enabled on the SYSTEM PARAMETERS screen see Section C 6 5 the schedule is automatically put on hold when it reaches the wait step in a sample prep p...

Page 289: ...Direct Control from the Front Panel NOTE This section describes how to control the AS directly from the front panel Local mode To run under direct control from the computer Locked Remote mode refer to...

Page 290: ...meters press Select or Select to select the desired setting TTL and relay fields Select 1 for on or 0 for off Inject valve s Select L to set the valve to load or I to set the valve to inject 6 After s...

Page 291: ...d events Section D 5 5 Parameters for each phase are defined on separate screens A method definition always includes the parameters from all three of the screens Notes The method phases are run in the...

Page 292: ...r details about selecting method setup parameters see Section D 5 4 For details about defining timed events see Section D 5 5 5 Save the method see Section D 5 2 D 5 2 Saving a Method 1 From any of th...

Page 293: ...pecifies for how long the AS waits before proceeding to the next step in the sample prep phase NEEDLE The height of the needle above the bottom of the vial PIPET Moves a specified volume of sample fro...

Page 294: ...e of tray installed NOTE To sample from a well plate the AS must be under Chromeleon or Chromeleon Xpress control To enter a relative position press Select or Select to scroll through the choices SMP...

Page 295: ...operation NOTE To delete a sample prep step position the cursor in the step s leftmost column and press Delete twice Using the Wait Operation The wait operation puts a method on hold until the AS rece...

Page 296: ...below wait and press the Insert button Enabling and Disabling the Wait Operation 1 If you are editing a method save the current settings see Section D 5 2 2 Go to the MODULE SETUP menu and press 5 to...

Page 297: ...sured from the tip of the needle to the bottom of the vial Zero is closest to the bottom of the vial 46 is closest to the top of the vial NOTE For 0 3 mL and 1 5 mL vials use a maximum needle height o...

Page 298: ...vial by repeatedly drawing up and expelling a volume of the contents The prep syringe is used if the sample preparation option is installed otherwise the sample syringe is used When specifying the vo...

Page 299: ...Reservoir DISPENSE is available only if the sample preparation option is installed During dispensing the prep syringe moves a volume of reagent from a reservoir to a vial Volume to Flush Through Needl...

Page 300: ...le syringe mixes the destination vial five times using 80 of the volume in the vial The sample syringe is flushed with a volume equal to the amount of concentrate plus one full syringe stroke 12 3 123...

Page 301: ...g reagent prime Optional The inject valve is switched to a specified position load or inject For most applications the valve position is not changed The needle arm moves to the waste port The prep syr...

Page 302: ...oves to the inject port The syringe delivers the specified volume of reagent from the reservoir to the inject port From there it moves onto the concentrator The sample syringe and the flush reservoir...

Page 303: ...s To go to the METHOD SETUP screen 1 Go to the METHOD MENU 2 Position the cursor on the 2 field SETUP 3 Press Enter Figure D 8 shows the default setup parameters Sample Syringe Volume L Reagent Prime...

Page 304: ...s Position in the Vial The SAMPLE NEEDLE HEIGHT parameter positions the tip of the needle at the specified distance above the bottom of the vial This height is always used for sample injections It is...

Page 305: ...ld displays tray temperature control is currently turned off Press Select or Select to turn on tray temperature control 3 Press numeric buttons to enter the desired temperature setting 4 Press Enter W...

Page 306: ...ield TIMED EVENTS 3 Press Enter Figure D 9 shows the default screen Figure D 9 Timed Events Screen Default Figure D 10 Timed Events Screen Simultaneous Injection Option Help Prompt METHOD TIMED EVENTS...

Page 307: ...lapsed since the previous injection see Section 2 7 5 for details Adding Timed Events Steps 1 Use one of the following methods to add a step Move the cursor to the empty TIME field below the last step...

Page 308: ...ss Delete To move the LOAD or INJECT parameter to a different step position the cursor in the VALVE field of the desired step and press Select or Select to select LOAD or INJECT Notes If you move the...

Page 309: ...nd edit the parameters as required 4 Save the method see Section D 5 2 The changes can be saved to the same method number or to a new unused method number If you save to a new number the original meth...

Page 310: ...defines injection parameters for each vial to be sampled A schedule consists of the following parameters The vials to sample The number of injections to draw from each vial The volume of each injectio...

Page 311: ...IT field Enter the number of an unused schedule or press Select or Select to scroll through the choices 1 through 9 3 Press Enter 4 Select an action to take if a vial is missing see Section D 6 3 5 De...

Page 312: ...e AS checks that a vial is present in the scheduled position in the tray If the vial is missing the AS can either stop and wait or skip the vial and continue The action is specified in the schedule 1...

Page 313: ...D 13 Schedule Screen Example Inserting Steps Position the cursor in the LINE field below where you want the new step to appear and press Insert Notes The new step is inserted above the cursor The STA...

Page 314: ...NJ VIAL Enter the number of injections 0 to 99 to perform on each vial If you enter 0 only the sample prep phase of the method is run If you enter multiple injections per vial the sample prep phase of...

Page 315: ...r If you save to a new number the original method remains unchanged D 6 6 Deleting a Schedule 1 Go to the SCHEDULE screen 2 Position the cursor in the EDIT field and press Delete A confirmation messag...

Page 316: ...d in the software see Section 3 7 Two syringe speed parameters are available METHOD SYRINGE SPEED and FLUSH SYRINGE SPEED The METHOD SYRINGE SPEED is used for the following operations Aspirating and d...

Page 317: ...setting for more viscous samples In addition be sure to select a speed that will keep the backpressure below 690 kPa 100 psi taking into account the pressure from the tubing and the concentrator colum...

Page 318: ...eedle seal assembly or if the needle becomes misaligned at anytime NOTE Before aligning the sampling needle always inspect the needle to make sure it is not bent If the needle is bent replace the samp...

Page 319: ...________ 5 Verify that the cursor is in the X ADDRESS field and press Enter The needle arm moves to the inject port 6 Move the cursor to the Z ADDRESS field and enter 180 Press Enter The needle moves...

Page 320: ...ecrease the Y ADDRESS value 9 After each adjustment move the cursor to the Z ADDRESS field keep the value at 180 and press Enter The needle arm moves back down Observe the alignment and adjust the pos...

Page 321: ...27 Repeat the FILL and EMPTY actions until the new needle seal tubing is filled 28 Once the tubing is filled and fluid reaches the needle empty the syringe again and monitor the top of the inject port...

Page 322: ...e the needle will hit the bottom of the port during operation and can damage the needle or port 30 When all adjustments are complete press Home 31 Factory calibration settings are recorded on a label...

Page 323: ...inject port volume manually using the front panel screens Dionex recommends manual calibration only if Chromeleon or Chromeleon Xpress Release 6 8 SP2 or later is unavailable Note for Sequential Inje...

Page 324: ...u 8 and 5 to go to the LIQUID CONTROL screen 3 Select VIAL INJ and press Enter The needle arm moves to the inject port NOTE Throughout the procedure retain the current or default settings for paramete...

Page 325: ...and press Enter The needle arm moves to the flush port 10 Select ACTION EMPTY and press Enter 11 Select the following options from FLUSH SYRINGE SPEED 5 ACTION FILL 12 Press Enter 13 Select the follow...

Page 326: ...d the volume entered including the volume in Step 17 and Step 19 Also notice the size of the liquid drop This will be compared to the size of the liquid drop in Step 34 22 Select the following options...

Page 327: ...E 33 Press Enter 34 Verify that a drop of liquid appears at the end of the needle seal tubing If no drop appears or if the drop is a different size than previously observed repeat Step 7 through Step...

Page 328: ...autosampler compartment door Record the new inject port volume on the label 44 If you are performing this procedure after replacing the needle seal assembly a Slide the bolt and ferrule removed from t...

Page 329: ...ess of 200 mA or with included power supplies over 60 V may damage the relay drivers on the CPU 1 2 3 4 5 6 7 8 9 10 11 12 TTL OUT TTL GND TTL IN 1 2 1 2 1 2 3 4 1 Pin Function Connector Position 1 2...

Page 330: ...t P N 062380 or P N 062381 2 Follow these basic steps to connect the TTL or relays a For each relay or TTL to be used connect an active wire red and a ground wire black to the 12 position connector pl...

Page 331: ...modules If the device connected to the AS does not send a normal edge signal select the appropriate control type Refer to the documentation provided with the controlling device and the information be...

Page 332: ...the positive and negative edges are reversed in function Normal Pulse In normal pulse operation the negative falling edge of the TTL signal is the active edge and the positive rising edge is ignored A...

Page 333: ...puts see Section E 1 turn on and off the output states in Direct Control from the DETAIL screen see Figure E 4 or in Schedule Control during the timed events portion of the method You can also use Chr...

Page 334: ...operator s manual for an example of how to set up the AS and the IC system for stand alone operation operation without Chromeleon or Chromeleon Xpress software Figure E 5 Example TTL Relay Connections...

Page 335: ...nd two well plate covers not suitable for IC applications 066325 Deep well plate tray and two well plates with 96 2 mL wells and two well plate covers suitable for IC applications where concentration...

Page 336: ...Syringe 5 mL standard for prep syringe 055068 Syringe 10 mL Tubing and Fittings 063405 PEEK tubing Violet 0 18 mm 0 007 in ID 042690 PEEK tubing Black 0 25 mm 0 010 in ID 049714 PEEK tubing Blue 0 33...

Page 337: ...1 4 28 x 1 8 in flangeless for sampling needle fitting 064553 Ferrule fitting 1 4 28 for sampling needle fitting Kits 057896 Valve Rebuild Kit for injection or diverter valves 062578 Second AS Kit for...

Page 338: ...AS Autosampler Operator s Manual 326 Doc 065051 03 1 08...

Page 339: ...sample prep syringe 108 An illegal vial number was specified 111 An injection was skipped because of a problem in the autosampler 123 Anion example QNT Method 225 Arrow buttons 11 Aspirate syringe sp...

Page 340: ...mode 24 62 82 Adjusting the syringe speed 304 Example program 85 Injection sequence 82 Reagent flush 82 240 Reagent prime 82 240 Setting up injections 83 Concentrator column 1 8 24 Installing 83 Loadi...

Page 341: ...Dionex Contacting 127 Direct control mode 27 Description of 27 Selecting 232 277 With Chromeleon Chromeleon Xpress 30 Disabling the wait operation 35 284 Dispense syringe speed 94 Dispensing reagents...

Page 342: ...detected 122 Cannot run schedule No sample syringe detected 122 Cannot use sample syringe to aspirate from specified source reservoir 111 Current leak sensor voltage is outside of legal calibration r...

Page 343: ...Example program Concentrate mode 85 Normal mode 72 Sequential mode 89 Simultaneous mode 80 Extractions 16 F Facility requirements 179 Factory set default 55 Filling a new syringe 134 Firmware 105 Fit...

Page 344: ...a schedule 275 Home position 10 262 Humidity operating 175 I ICS 1000 1500 2000 systems Connections for simultaneous injections 193 195 Example program for simultaneous injections 81 Injection valve...

Page 345: ...46 302 Zero 299 Inserting lines in a method 10 Inserting steps in a schedule 10 301 Installation 179 AS with ICS 3000 system 204 Connecting a second AS 200 Diverter valve connections 197 Dual AS conne...

Page 346: ...inges valves and needle arm 264 Matrix elimination 82 85 90 Maximum loop and injection volumes 68 69 Maximum number of steps reached 105 Memory is full 106 Menu button 11 Menu of Screens 13 228 Select...

Page 347: ...are connected 122 Non recoverable motion error occurred 114 Normal edge TTL input control Definition 319 Selecting 259 319 Normal mode 62 Calibrating the inject port volume 157 Example program 72 80...

Page 348: ...ation screen 249 Selecting options 210 Polymer vials 19 PolyVial 19 Port Flush 19 Inject 19 Waste 19 Power 206 Specifications 175 Power cord 206 Power receptacle 26 206 Power switch 7 8 Power Up scree...

Page 349: ...Removing Bubbles from the syringe 134 218 Drip tray 145 Sample or prep syringe valve 164 Syringes 133 Renaming devices 220 Renaming signals 222 Reordering parts 323 Replacing Leak sensor 161 Needle s...

Page 350: ...2 Specifying the volume of 210 250 Valve port flow schematics 43 Volumes of 15 Sample temperature control option 8 22 Installation 188 Specifications 177 Tray covers for 46 Tray for 19 Using with well...

Page 351: ...rvice 127 Aligning the inject port 151 Aligning the sampling needle 151 Calibrating the inject port volume 155 Calibrating the leak sensor 162 Changing the main power fuses 173 Installing the drip tra...

Page 352: ...54 Selecting 92 93 Selecting for front panel operation 304 305 Syringes 8 15 Connecting the liquid lines 187 Filling 134 Initializing 135 Manually controlling 264 Prep syringe description of 16 Remov...

Page 353: ...05 Liquid leaks 124 TTL inputs Control of 27 259 318 Function assignments 259 318 TTL signal mode 259 TTL relay connector 26 317 Connection instructions 318 Pin assignments 317 TTL relay control Direc...

Page 354: ...inge 210 250 W Wait For Temp Stable option 36 243 293 Wait operation 240 281 283 Changing the position in the method 284 Cycle time and 36 Disabling 35 252 284 Enabling 252 284 Overview of 33 Waiting...

Page 355: ...Index Doc 065051 03 1 08 Index 17 Z Z address 255...

Page 356: ...AS Autosampler Operator s Manual Index 18 Doc 065051 03 1 08...

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